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Imaging and Ion-Beam Milling of Biological Specimens with the Helium-Ion Microscope

Published online by Cambridge University Press:  30 July 2021

Matthias Schmidt ,
Chaturanga Bandara ,
Marc Tamisier ,
Ilari Maasilta  and
James Byrne
Matthias Schmidt
Affiliation:
Helmholtz-Centre for Environmental Research GmbH - UFZ, Leipzig, Sachsen, Germany
Chaturanga Bandara
Affiliation:
Helmholtz-Centre for Environmental Research GmbH - UFZ, Leipzig, Sachsen, Germany
Marc Tamisier
Affiliation:
Helmholtz-Centre for Environmental Research GmbH - UFZ, Leipzig, Sachsen, Germany
Ilari Maasilta
Affiliation:
University of Jyväskylä, Jyväskylä, Finland
James Byrne
Affiliation:
University of Bristol, Bristol, England, United Kingdom

Abstract

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Type
Advances in Focused Ion Beam Instrumentation, Applications and Techniques in and Materials and Life Sciences
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 768 - 769
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Schmidt, Matthias, Byrne, James and Maasilta, Ilari, Beilstein J. Nanotechnol. 2021, 12, DOI: 10.3762/bjnano.12.1CrossRefGoogle Scholar
Frese, Natalie, Schmerer, Patrick, Wortmann, Martin, Schürmann, Matthias, König, Matthias, Westphal, Michael, Weber, Friedemann, Sudhoff, Holger and Gölzhäuser, Armin, Beilstein J. Nanotechnol. 2021, 12, DOI: 10.3762/bjnano.12.13Google Scholar
The authors acknowledge scientific and technical assistance of Federica Calabrese, Musat, Niculina, Nerlich, Katja, Nedal Said and Hryhoriy Stryhanyuk at Helmholtz – Centre for Environmental Research GmbH – UFZ, ProVIS, Leipzig, GermanyGoogle Scholar
Wolff, A., Klingner, N., Thompson, W., Zhou, Y., Lin, J., Peng, Y.Y., Ramshaw, J.A.M. and Xiao, Y., J. Microscopy 2018, 272 (1), pp. 47-59, DOI: 10.1111/jmi.12731Google Scholar
The authors are grateful to Robin Dittrich for systematic ion-beam milling of wings collected from bees and dragonflies (no killing of insects, for this study only dead animals were collected) during his internship at Helmholtz – Centre for Environmental Research GmbH – UFZ, ProVIS, Leipzig, Germany.Google Scholar
Ziegler, J.F., Biersack, J.P. and Ziegler, M.D. 2015 SRIM – the stopping and range of ions in matter. SRIM Company. ISBN: 0-9654207-1-XGoogle Scholar
Moreno Osorio, Jairo H., Benettoni, Pietro, Schmidt, Matthias, Stryhanyuk, Hryhoriy, Schmitt-Jansen, Mechthild, Pinto, Gabriele, Pollio, Antonino, Frunzo, Luigi, Lens, Piet N.L., Richnow, Hans H., Esposito, Giovanni and Musat, Niculina, FEMS Microbiology Ecology 2019, 95, fiz029, DOI: 10.1093/femsec/fiz029Google Scholar
The authors are thankful for the use of the helium-ion microscope at the Centre for Chemical Microscopy (ProVIS) at UFZ Leipzig, which is supported by European Regional Development Funds (EFRE-Europe funds Saxony) and the Helmholtz Association, and for the use of the helium-ion microscope at University of Jyväskylä, which is funded by the Academy of Finland within the FIRI2014 programme.Google Scholar