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Human Bloodstains on Biological Materials: High-Vacuum Scanning Electron Microscope Examination Using Specimens without Previous Preparation

Published online by Cambridge University Press:  04 February 2013

Policarp Hortolà
Policarp Hortolà*
Affiliation:
Àrea de Prehistòria, Universitat Rovira i Virgili, Avinguda de Catalunya 35, ES-43002 Tarragona, Catalonia, Spain Institut Català de Paleoecologia Humana i Evolució Social, Carrer de Marcel·lí Domingo s/n, Edifici W3 Campus Sescelades, ES-43007 Tarragona, Catalonia, Spain
*
*Corresponding author. E-mail: [email protected]
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Abstract

Studies of human bloodstains on nonbiological materials have been previously carried out using a high-vacuum scanning electron microscope (HV-SEM) in secondary-electron mode without any sample treatment. To assess whether biological substrates can affect the morphology of human erythrocytes in bloodstains, three fragments of different biological material (bone, shell, and wood) were smeared with peripheral human blood. Afterward, the bloodstains were directly examined in secondary-electron mode by an HV-SEM following a procedure initially standardized to be used in uncoated human bloodstains on stone. The obtained results suggest that HV-SEM is suitable for examining untreated bloodstains on biological substrate and that the morphology of erythrocytes in human bloodstains is not affected by the biological nature of the substrate. A cautionary issue regarding bloodstains on nondehydrated biological substrates is that the waiting time required for initiating the HV-SEM examination is by far higher than when using inorganic bloodstain substrates.

Keywords

blood smearsred blood cellshaemotaphonomyscanning electron microscopyspecimen preparationbioimaging
Type
Biological Applications: Short Communications
Information
Microscopy and Microanalysis , Volume 19 , Issue 2 , April 2013 , pp. 415 - 419
Copyright
Copyright © Microscopy Society of America 2013

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References

Gerlach, S.C., Newman, M., Knell, E.J. & Hall, E.S. Jr. (1996). Blood protein residues on lithic artifacts from two archaeological sites in the De Long Mountains, northwestern Alaska. Arctic 49, 1–10.CrossRefGoogle Scholar
Goldstein, J.I., Newbury, D.E., Echlin, P., Joy, D.C., Lyman, C.E., Lifshin, E., Sawyer, L. & Michael, J.R. (2003). Scanning Electron Microscopy and X-Ray Microanalysis, 3rd ed., vol. 1. New York: Springer.CrossRefGoogle Scholar
Gramly, R.M. (1991). Blood residues upon tools from the East Wenatchee Clovis site, Douglas County, Washington. Ohio Archaeol 41, 4–9.Google Scholar
Holdgate, M.W. (1961). Man and environment in the south Chilean islands. Geogr J 127, 401–414.CrossRefGoogle Scholar
Hortolà, P. (2002). Red blood cell haemotaphonomy of experimental human bloodstains on techno-prehistoric lithic raw materials. J Archaeol Sci 29, 733–739.CrossRefGoogle Scholar
Hortolà, P. (2005). SEM examination of human erythrocytes in uncoated bloodstains on stone: Use of conventional as environmental-like SEM in a soft biological tissue (and hard inorganic material). J Microsc 218, 94–103.CrossRefGoogle Scholar
Hortolà, P. (2006). Using an SEM as an ESEM to study minute human bloodstains on stainless steel. Microsc Anal 20(6), 15–17 (UK), 5–7 (EU), 23–25 (US), 11–13 (AP). Google Scholar
Hortolà, P. (2008). Secondary-electron SEM bioimaging of human erythrocytes in bloodstains on high-carbon steel substrate without specimen preparation. Micron 39, 53–55.CrossRefGoogle ScholarPubMed
Jones, P.J. (2009). A microstratigraphic investigation into the longevity of archaeological residues, Sterkfontein, South Africa. In Archaeological Science under a Microscope. Studies in Residue and Ancient DNA Analysis in Honour of Thomas H. Loy, Haslam, M., Robertson, G., Crowther, A., Nugent, S. & Kirkwood, L. (Eds.), pp. 29–46. Canberra, Australia: ANU E Press.Google Scholar
Kononenko, N., Bedford, S. & Reepmeyer, C. (2010). Functional analysis of late Holocene flaked and pebble stone artefacts from Vanuatu, Southwest Pacific. Archaeol Oceania 45, 13–20.CrossRefGoogle Scholar
Lawes, G. (1987). Scanning Electron Microscopy and X-ray Microanalysis. Chichester, UK: John Wiley & Sons.Google Scholar
LeBlond, P.F. & Shoucri, R. (1978). Calculation of surface area and volume of human erythrocytes from scanning electron micrographs. J Microsc 113, 161–170.CrossRefGoogle ScholarPubMed
Lombard, M. (2005). Evidence of hunting and hafting during the Middle Stone Age at Sibidu Cave, KwaZulu-Natal, South Africa: A multianalytical approach. J Hum Evol 48, 279–300.CrossRefGoogle ScholarPubMed
Lombard, M. (2008). Finding resolution for the Howiesons Poort through the microscope: Micro-residue analysis of segments from Sibudu Cave, South Africa. J Archaeol Sci 35, 26–41.CrossRefGoogle Scholar
Loy, T.H. & Dixon, E.J. (1998). Blood residues on fluted points from eastern Beringia. Am Antiq 63, 21–46.CrossRefGoogle Scholar
Loy, T.H. & Hardy, B.L. (1992). Blood residue analysis of 90,000-year-old stone tools from Tabun Cave, Israel. Antiquity 66, 24–35.CrossRefGoogle Scholar
Lyons, A.P. (1922). The arrows of the Upper Morehead River (Papua) bush tribes. Man 22, 145–147 + Pl. K. CrossRefGoogle Scholar
Mazel, V., Richardin, P., Debois, D., Touboul, D., Cotte, M., Brunelle, A., Walter, P. & Laprévote, O. (2007). Identification of ritual blood in African artifacts using TOF-SIMS and synchrotron radiation microspectroscopies. Anal Chem 79, 9253–9260.CrossRefGoogle ScholarPubMed
Melki, J.A.D., Martin, C.C.S. & Zerbini, T. (2011). Scanning electron microscopy as an auxiliary method in the study of exhumed bones [Case report]. Forensic Sci Int 206, e67–e70.CrossRefGoogle Scholar
Robertson, G., Attenbrow, V. & Hiscock, P. (2009). Multiple uses for Australian backed artefacts. Antiquity 83, 296–308.CrossRefGoogle Scholar
Rots, V. & Williamson, B.S. (2004). Microwear and residue analyses in perspective: The contribution of ethnoarchaeological evidence. J Archaeol Sci 31, 1287–1299.CrossRefGoogle Scholar
Seeman, M.F., Nilsson, N.E., Summers, G.L., Morris, L.L., Barans, P.J., Dowd, E. & Newman, M.E. (2008). Evaluating protein residues on Gainey phase Paleoindian stone tools. J Archaeol Sci 35, 2742–2750.CrossRefGoogle Scholar
Thieme, H. (1997). Lower Palaeolithic hunting spears from Germany. Nature 385, 807–810.CrossRefGoogle ScholarPubMed
Torrence, R. (1993). Ethnoarchaeology, museum collections and prehistoric exchange: Obsidian-tipped artifacts from the Admiralty Islands. World Archaeol 24, 467–481.CrossRefGoogle Scholar
Wadley, L., Lombard, M. & Williamson, B. (2004). The first residue analysis blind tests: Results and lessons learnt. J Archaeol Sci 31, 1491–1501.CrossRefGoogle Scholar
Zhilin, M.G. (1998). Technology of the manufacture of Mesolithic bone arrowheads on the Upper Volga. Eur J Archaeol 1, 149–176.CrossRefGoogle Scholar