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Green Tea Infusion Ameliorates Histological Damages in Testis and Epididymis of Diabetic Rats

Published online by Cambridge University Press:  29 June 2021

Luiz Otávio Guimarães-Ervilha ,
Luiz Carlos Maia Ladeira ,
Renner Philipe Rodrigues Carvalho ,
Isabela Pereira da Silva Bento ,
Daniel Silva Sena Bastos ,
Ana Cláudia Ferreira Souza Open the ORCID record for Ana Cláudia Ferreira Souza [Opens in a new window] ,
Eliziária Cardoso Santos ,
Leandro Licursi de Oliveira ,
Izabel Regina dos Santos Costa Maldonado  and
Mariana Machado-Neves Open the ORCID record for Mariana Machado-Neves [Opens in a new window]
Luiz Otávio Guimarães-Ervilha
Affiliation:
Department of General Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
Luiz Carlos Maia Ladeira
Affiliation:
Department of General Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
Renner Philipe Rodrigues Carvalho
Affiliation:
Department of General Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
Isabela Pereira da Silva Bento
Affiliation:
Department of General Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
Daniel Silva Sena Bastos
Affiliation:
Department of General Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
Ana Cláudia Ferreira Souza
Affiliation:
Department of Animal Biology, Universidade Federal Rural do Rio de Janeiro, Rio de Janeiro23897-000, Brazil
Eliziária Cardoso Santos
Affiliation:
Medicine School, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Minas Gerais39100-000, Brazil
Leandro Licursi de Oliveira
Affiliation:
Department of General Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
Izabel Regina dos Santos Costa Maldonado
Affiliation:
Department of General Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
Mariana Machado-Neves*
Affiliation:
Department of General Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
*
*Author for correspondence: Mariana Machado-Neves, E-mail: [email protected]
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Abstract

Green tea is a popular drink used for therapeutic purposes to mitigate the consequences of diabetes. In this study, we aimed at evaluating the potential of green tea infusion to ameliorate structural and enzymatic damages caused by hyperglycemia in the testis and epididymis of Wistar rats. For that, nondiabetic and streptozotocin-induced diabetic rats (negative control and diabetes control, respectively) received 0.6 mL of water by gavage. Another set of diabetic animals received 100 mg/kg of green tea infusion diluted in 0.6 mL of water/gavage (diabetes + green tea) daily. After 42 days of treatment, the testes and epididymides were removed and processed for histopathological analysis, micromineral determination, and enzymatic assays. The results showed that treatment with green tea infusion preserved the testicular and epididymal histoarchitecture, improving the seminiferous epithelium and the sperm production previously affected by diabetes. Treatment with green tea reduced tissue damages caused by this metabolic condition. Given the severity of hyperglycemia, there was no efficacy of the green tea infusion in maintaining the testosterone levels, antioxidant enzyme activity, and microminerals content. Thus, our findings indicate a protective effect of this infusion on histological parameters, with possible use as a complementary therapy for diabetes.

Keywords

antioxidant defenseCamellia sinensisepididymal ducthyperglycemiapathologysperm production
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 27 , Issue 5 , October 2021 , pp. 1133 - 1145
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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