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The Good, the Bad and the Ugly: Task-Specific Fixation for Connective Tissues

Published online by Cambridge University Press:  30 July 2021

Douglas Keene  and
Sara Tufa
Douglas Keene
Affiliation:
Shriners Hospital for Childern, Portland, Oregon, United States
Sara Tufa
Affiliation:
Shriners Hospital for Childern, Portland, Oregon, United States

Abstract

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Type
To Fix or Not To Fix? A Question for Biological Samples
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 1402 - 1403
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Keene, DR and Tufa, SF (2020). Connective tissue ultrastructure: A direct comparison between conventional specimen preparation and high-pressure freezing/freeze substitution. Anat Rec (Hoboken) 303:1514-1526.CrossRefGoogle ScholarPubMed
Hunziker, EB, Ludi, A, Herrmann, W (1992). Preservation of cartilage matrix proteoglycans using cationic dyes chemically related to ruthenium hexamine trichloride. J Histochem Cytochem 40:909-917.Google Scholar
Keene, DR and Tufa, SF (2010). Transmission electron microscopy of cartilage and bone. Meth Cell Biol 96:443-473.Google ScholarPubMed
Keene, DR and Tufa, SF (2018). Ultrastructural analysis of the extracellular matrix. Methods Cell Biol 143:1-39.Google ScholarPubMed