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A Gill Histopathology Study in two Native Fish Species from the Hydrographic Douro Basin

Published online by Cambridge University Press:  12 February 2019

Dércia Santos ,
Ana Luzio ,
Ana M. Coimbra Open the ORCID record for Ana M. Coimbra [Opens in a new window] ,
Simone Varandas ,
António Fontaínhas-Fernandes  and
Sandra M. Monteiro
Dércia Santos*
Affiliation:
Departamento de Biologia e Ambiente (DeBA), Centro de Investigação de Tecnologias Agro-Ambientais e Biológicas (CITAB), Escola de Ciências da Vida e Ambiente (ECVA), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
Ana Luzio
Affiliation:
Departamento de Biologia e Ambiente (DeBA), Centro de Investigação de Tecnologias Agro-Ambientais e Biológicas (CITAB), Escola de Ciências da Vida e Ambiente (ECVA), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
Ana M. Coimbra
Affiliation:
Departamento de Biologia e Ambiente (DeBA), Centro de Investigação de Tecnologias Agro-Ambientais e Biológicas (CITAB), Escola de Ciências da Vida e Ambiente (ECVA), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
Simone Varandas
Affiliation:
Departamento de Ciências Florestais e Arquitetura Paisagista (CIFAP), Centro de Investigação de Tecnologias Agro-Ambientais e Biológicas (CITAB), Escola de Ciências Agrárias e Veterinárias (ECAV), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
António Fontaínhas-Fernandes
Affiliation:
Departamento de Biologia e Ambiente (DeBA), Centro de Investigação de Tecnologias Agro-Ambientais e Biológicas (CITAB), Escola de Ciências da Vida e Ambiente (ECVA), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
Sandra M. Monteiro*
Affiliation:
Departamento de Biologia e Ambiente (DeBA), Centro de Investigação de Tecnologias Agro-Ambientais e Biológicas (CITAB), Escola de Ciências da Vida e Ambiente (ECVA), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
*
*Authors for correspondence: Dércia Santos, E-mail: [email protected]; Sandra M. Monteiro, E-mail: [email protected]
*Authors for correspondence: Dércia Santos, E-mail: [email protected]; Sandra M. Monteiro, E-mail: [email protected]
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Abstract

Gill histopathology is a valuable tool to evaluate ecosystems health, because the gill is a key site of waterborne pollutant uptake and the first target organ to their toxicity. Consequently, this makes it important in biomonitoring programs. This study aims to evaluate gill histopathological differences in Douro basin native fish species and determine possible associations with water quality and the ecological status classifications. Two native fish species (Pseudochondrostoma duriense and Luciobarbus bocagei) were sampled in four points of the Douro basin: two reference points, Ameixiosa (Paiva River) and Covelas (Bestança River), both classified with an excellent ecological status; and two disturbed points, Castro Daire (Paiva River) and Alvações do Corgo (Corgo River), categorized with a good and a moderate ecological status, respectively. Gill histopathological differences were qualitative and quantitatively analyzed. The histological analysis showed that, in all sampling locations, both species presented some degree of gill differences, such as epithelial lifting, lamellar fusion, and/or necrosis. The histopathological differences evaluation emphasized some variances in the responses between the two species. In nase, the filament and lamellar epithelium proliferation were the histopathological differences that better reflected the river ecological status classification, proving their usefulness in biomonitoring programs.

Keywords

biomonitoringfreshwater fisheshistologyLuciobarbus bocageiPseudochondrostoma duriense
Type
Life Sciences
Information
Microscopy and Microanalysis , Volume 25 , Issue 1 , February 2019 , pp. 236 - 243
Copyright
Copyright © Microscopy Society of America 2019 

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