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Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity

Published online by Cambridge University Press:  09 February 2016

Aleksandar Savić ,
Aleksandra Mitrović ,
Lloyd Donaldson ,
Jasna Simonović Radosavljević ,
Jelena Bogdanović Pristov ,
Gabor Steinbach ,
Győző Garab  and
Ksenija Radotić
Aleksandar Savić
Affiliation:
Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11000 Belgrade, Serbia Laboratoire de Spectrochimie Infrarouge et Raman—UMR 8516, Université de Lille, Sciences et Technologies, 59655 Villeneuve d’Ascq Cedex; France
Aleksandra Mitrović
Affiliation:
Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11000 Belgrade, Serbia
Lloyd Donaldson
Affiliation:
Scion, Private Bag 3020, Rotorua 3010, New Zealand
Jasna Simonović Radosavljević
Affiliation:
Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11000 Belgrade, Serbia
Jelena Bogdanović Pristov
Affiliation:
Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11000 Belgrade, Serbia
Gabor Steinbach
Affiliation:
Institute of Plant Biology, Biological Research Center, H-6701 Szeged, Hungary Institute of Microbiology, CAS, Centrum Algatech, 379 01 Třeboň, Czech Republic
Győző Garab
Affiliation:
Institute of Plant Biology, Biological Research Center, H-6701 Szeged, Hungary
Ksenija Radotić*
Affiliation:
Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11000 Belgrade, Serbia
*
*Corresponding author.[email protected]
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Abstract

Fluorescence-detected linear dichroism (FDLD) microscopy provides observation of structural order in a microscopic sample and its expression in numerical terms, enabling both quantitative and qualitative comparison among different samples. We applied FDLD microscopy to compare the distribution and alignment of cellulose fibrils in cell walls of compression wood (CW) and normal wood (NW) on stem cross-sections of juvenile Picea omorika trees. Our data indicate a decrease in cellulose fibril order in CW compared with NW. Radial and tangential walls differ considerably in both NW and CW. In radial walls, cellulose fibril order shows a gradual decrease from NW to severe CW, in line with the increase in CW severity. This indicates that FDLD analysis of cellulose fibril order in radial cell walls is a valuable method for estimation of CW severity.

Keywords

cell wallcellulose fibrilscompression wood severityfluorescence-detected linear dichroism microscopyPicea omorika (Pančić) Purkynĕ
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 22 , Issue 2 , April 2016 , pp. 361 - 367
Copyright
© Microscopy Society of America 2016 

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