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Flow Cytometry and Electron Microscopy Study of Staphylococcus aureus and Escherichia coli Treated with Mdc-Hly

Published online by Cambridge University Press:  13 March 2015

Xuemei Lu ,
Xiaobao Jin  and
Jiayong Zhu
Xuemei Lu
Affiliation:
School of Basic Courses, Guangzhou Higher Education Mega Center, Guangdong Pharmaceutical University, 280 Wai Huan Dong Road, Guangzhou, Guangdong 510006, People’s Republic of China Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou Higher Education Mega Center, 280 Wai Huan Dong Road, Guangzhou, Guangdong 510006, People’s Republic of China
Xiaobao Jin
Affiliation:
School of Basic Courses, Guangzhou Higher Education Mega Center, Guangdong Pharmaceutical University, 280 Wai Huan Dong Road, Guangzhou, Guangdong 510006, People’s Republic of China Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou Higher Education Mega Center, 280 Wai Huan Dong Road, Guangzhou, Guangdong 510006, People’s Republic of China
Jiayong Zhu*
Affiliation:
School of Basic Courses, Guangzhou Higher Education Mega Center, Guangdong Pharmaceutical University, 280 Wai Huan Dong Road, Guangzhou, Guangdong 510006, People’s Republic of China Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou Higher Education Mega Center, 280 Wai Huan Dong Road, Guangzhou, Guangdong 510006, People’s Republic of China
*
*Corresponding author.[email protected]
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Abstract

In our previous study, a novel hybrid protein combining human lysozyme (Hly) with Musca domestica cecropin (Mdc) was successfully constructed. The broad antibacterial activity against various foodborne pathogens of Mdc-hly suggests its scope as a food preservative. The aim of the present study was to investigate the antibacterial mechanism of the recombinant Mdc-hly. The damage induced by Mdc-hly on Staphylococcus aureus and Escherichia coli was investigated using flow cytometry (FC), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The results of FC showed that Mdc-hly causes bacterial membrane permeabilization. SEM and TEM studies revealed that Mdc-hly is capable of damaging both the membrane and the wall of bacteria, resulting in efflux of essential cytoplasmic contents. Both FC and EM revealed that the effects of Mdc-hly were greater than its parental peptides. Understanding the antibacterial mechanism of Mdc-hly is of a great interest in further utilization of its use in treatment of food and in clinical environments.

Keywords

Musca domestica cecropinhuman lysozymefusion proteinflow cytometryelectron microscopy
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 21 , Issue 2 , April 2015 , pp. 351 - 357
Copyright
© Microscopy Society of America 2015 

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