Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-25T16:17:09.031Z Has data issue: false hasContentIssue false
  • English
  • Français

FIB-SEM: An Additional Technique for Investigating Internal Structure of Pollen Walls

Published online by Cambridge University Press:  28 August 2013

Alisoun House  and
Kevin Balkwill
Alisoun House*
Affiliation:
School of Animal Plant and Environmental Sciences, University of the Witwatersrand, P.O. WITS 2050, Johannesburg, South Africa
Kevin Balkwill
Affiliation:
School of Animal Plant and Environmental Sciences, University of the Witwatersrand, P.O. WITS 2050, Johannesburg, South Africa
*
*Corresponding author. E-mail: [email protected]
Get access

Abstract

Pollen grain morphology has been widely used in the classification of the Acanthaceae, where external pollen wall features have proved useful in determining relationships between taxa. Although detailed information has been accumulated using light microscopy, transmission electron microscopy and scanning electron microscopy (SEM) techniques, internal pollen wall features lack investigation and the techniques are cumbersome. A new technique involving precise cross sectioning or slicing of pollen grains at a selected position for examining wall ultrastructure, using a focused ion beam-scanning electron microscope (FIB-SEM), has been explored and promising results have been obtained. The FIB-SEM offers a good technique for reliable, high resolution, three-dimensional (3D) viewing of the internal structure of the pollen grain wall.

Keywords

Acanthaceaeexinefocused ion beam-scanning electron microscopeFIB-SEMpalynologypollen wall ultrastructure
Type
Biomedical and Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 6 , December 2013 , pp. 1535 - 1541
Copyright
Copyright © Microscopy Society of America 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Balkwill, K. & Getliffe Norris, F. (1988). Classification of the Acanthaceae: A southern African perspective. Monogr Syst Bot Missouri Bot Gard 25, 503–516.Google Scholar
Bremekamp, C.E.B. (1953). The delimitation of the Acanthaceae. Proc Kon Ned Akad Wetensch Ser. C 56, 533–546.Google Scholar
Bremekamp, C.E.B. (1965). Delimitation and subdivision of the Acanthaceae. Bull Bot Surv India 7, 21–30.Google Scholar
Daniel, T.F. (1978). A new Mirandea (Acanthaceae) from Nuevo Leon, Mexico. Syst Bot 6, 288–293.Google Scholar
Daniel, T.F. (1986). Systematics of Tetramerium (Acanthaceae). Syst Bot Monogr 12, 1–134.CrossRefGoogle Scholar
Daniel, T.F. (1990). New and reconsidered Mexican Acanthaceae. IV. Proc Calif Acad Sci 46, 279–287.Google Scholar
Daniel, T.F. (1998). Pollen morphology of Mexican Acanthaceae: Diversity and systematic significance. Proc Calif Acad Sci 50(8), 217–256.Google Scholar
Furness, C.A. (1990). Pollen morphology of Crossandra Salisbury and Crossandrella C.B. Clarke (Acanthaceae: Acantheae). Grana 29, 161–176.CrossRefGoogle Scholar
Furness, C.A. (1991). Pollen morphology of Sclerochiton (Acanthaceae: Acantheae). Kew Bull 46, 51–59.Google Scholar
Furness, C.A. (1993). A pollen morphological survey of the Old World species of Stenandrium Nees (Acanthaceae: Acantheae). Grana 32, 1–11.Google Scholar
Furness, C.A. (1994). The pollen morphology of Hygrophila and Brillantaisia (Acanthaceae: Ruellieae). Acta Bot Gallica 141, 267–278.Google Scholar
Furness, C.A. (1996). Pollen morphology of Acanthopsis Harvey, Acanthus L. and Blepharis Jussieu (Acanthaceae: Acantheae). Rev Palaeobot Palynol 92, 253–268.Google Scholar
Graham, V.A.W. (1988). Delimitation and infra-generic classification of Justicia (Acanthaceae). Kew Bull 43, 551–624.CrossRefGoogle Scholar
Immelman, K.L. (1989). Studies in the southern African species of Justicia and Siphonoglossa (Acanthaceae): Palynology. Bothalia 19(2), 151–156.Google Scholar
Lindau, G. (1893). Beiträge zur Systematik der Acanthaceen. Bot Jahrb Syst 18, 36–64.Google Scholar
Lindau, G. (1895). Acanthaceae. In Die natuerlichen Pflanzenfamilien, vol. 4(3b), Engler, A. & Prantl, K. (Eds.), pp. 274–354. Leipzig: Wilhelm Engelmann.Google Scholar
McDade, L.A., Masta, S.E., Moody, M.L. & Waters, E. (2000). Phylogenetic relationships among Acanthaceae: Evidence from two genomes. Syst Bot 25(1), 106–121.Google Scholar
Moore, P.D., Webb, J.A. & Collinson, M.E. (1991). Pollen Analysis, 2nd ed., pp. 76–77. Oxford: Blackwell Science Ltd. Google Scholar
Perveen, A. & Qaiser, M. (2010). Pollen flora of Pakistan—LXVII: Acanthaceae. Pak J Bot 42, 175–191.Google Scholar
Raj, B. (1961). Pollen morphological studies in the Acanthaceae. Grana Palynol 3, 3–108.Google Scholar
Scotland, R.W. (1990). Homology, pollen grains and microscopy. Proc Royal Microscop Soc 25, 108.Google Scholar
Scotland, R.W. (1992). Systematics, similarity and Acanthaceae pollen morphology. Bot J Linn Soc 109, 529–541.Google Scholar
Scotland, R.W. (1993). Pollen morphology of Contortae (Acanthaceae). Bot J Linn Soc 111, 471–504.CrossRefGoogle Scholar
Scotland, R.W. & Vollesen, K. (2000). Classification of Acanthaceae. Kew Bull 55, 513–589.Google Scholar
Villanueva-Amadoz, U., Benedetti, A., Mendez, J., Sender, L.M. & Diez, J.B. (2012). Focused ion beam nano-sectioning and imaging: A new method in characterisation of palaeopalynological remains. Grana 51, 1–9.Google Scholar
Walker, J.W. & Doyle, J.A. (1975). The bases of angiosperm phylogeny: Palynology. Ann Missouri Bot Gard 62, 664–723.CrossRefGoogle Scholar