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Evaluation Of Diseased State In Human Tissue Sections Using Infrared And Raman Imaging Microspectroscopy

Published online by Cambridge University Press:  02 July 2020

E. Neil Lewis ,
Abigail S. Haka ,
Pina Colarusso ,
Ira W. Levin ,
John Gillespie  and
Linda H. Kidder
E. Neil Lewis
Affiliation:
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, Building 5, Room B1-38, National Institutes of Health, Bethesda, MD20892
Abigail S. Haka
Affiliation:
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, Building 5, Room B1-38, National Institutes of Health, Bethesda, MD20892
Pina Colarusso
Affiliation:
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, Building 5, Room B1-38, National Institutes of Health, Bethesda, MD20892
Ira W. Levin
Affiliation:
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, Building 5, Room B1-38, National Institutes of Health, Bethesda, MD20892
John Gillespie
Affiliation:
National Cancer Institute, National Institutes of Health, Building 10, Room 2C500, Bethesda, MD20892
Linda H. Kidder
Affiliation:
Spectral Dimensions Inc., 3403 Olandwood Ct.,Suite 102, Olney, MD20832
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Extract

Optical microscopy has been the workhorse pathological technique, qualitatively differentiating tissue sections by characterizing morphological variations. Vibrational spectroscopic techniques provide quantitative as well as qualitative analytical information that reflects a sample’s biochemical composition and molecular structure. The utility of infrared and Raman techniques for biological characterization has been demonstrated for a variety of applications.[1-4]

FTIR imaging microscopy is a newly developed technique that incorporates the imaging capabilities required for histological procedures with the chemical discrimination of IR spectroscopy.[5-7] The ability to maintain spatial integrity while accessing precise spectroscopic data intrinsic to the sample represents a powerful combination. This technique is much more amenable to analysis by a pathologist than conventional spectroscopy because the data can be presented as images. These images provide direct visualization of a sample’s biochemical heterogeneity.

Vibrational spectroscopic imaging techniques provide excellent sample statistics for the accurate classification of individual spectral signatures because tens of thousands of independent spectra from different spatial locations within the sample are simultaneously recorded.

Type
Optical Microanalysis Via Molecular Spectroscopy
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 60 - 61
Copyright
Copyright © Microscopy Society of America

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References

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