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Electron Holography Study of the Charging Effect in Microfibrils of Sciatic Nerve Tissues

Published online by Cambridge University Press:  06 August 2013

Ki Hyun Kim ,
Zentaro Akase ,
Daisuke Shindo ,
Nobuhiko Ohno ,
Yasuhisa Fujii ,
Nobuo Terada  and
Shinichi Ohno
Ki Hyun Kim
Affiliation:
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai 980-8577, Japan
Zentaro Akase
Affiliation:
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai 980-8577, Japan
Daisuke Shindo*
Affiliation:
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai 980-8577, Japan
Nobuhiko Ohno
Affiliation:
Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan
Yasuhisa Fujii
Affiliation:
Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan
Nobuo Terada
Affiliation:
Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan
Shinichi Ohno
Affiliation:
Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan
*
*Corresponding author. E-mail: [email protected]
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Abstract

The charging effects of microfibrils of sciatic nerve tissues due to electron irradiation are investigated using electron holography. The phenomenon that the charging effects are enhanced with an increase of electron intensity is visualized through direct observations of the electric potential distribution around the specimen. The electric potential at the surface of the specimen could be quantitatively evaluated by simulation, which takes into account the reference wave modulation due to the long-range electric field.

Keywords

electron holographysecondary electronselectric potential distributioncharging effectmicrofibrilphase reconstructionamplitude reconstruction
Type
Research Article
Information
Microscopy and Microanalysis , Volume 19 , Supplement S5: IUMAS , August 2013 , pp. 54 - 57
Copyright
Copyright © Microscopy Society of America 2013 

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Footnotes

Primary institution where the research was performed is the Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Japan

References

Jackson, J.D. (1998). Classical Electrodynamics, 3rd ed. New York, Chichester: John Wiley & Sons.Google Scholar
Kotera, M., Yamaguchi, K. & Suga, H. (1999). Dynamic simulation of electron-beam-induced charging up of insulators. Jpn J Appl Phys 38(12B), 7176–7179.10.1143/JJAP.38.7176Google Scholar
Li, D.J. & Gu, H.Q. (2002). Cell attachment on diamond-like carbon coating. Bull Mater Sci 25(1), 7–13.10.1007/BF02704587Google Scholar
Matteucci, G., Missiroli, G. & Pozzi, G. (1997). Simulations of electron holograms of long range electrostatic field. Scanning Microsc 11, 367–374.Google Scholar
Matteucci, G., Missiroli, G.F., Muccini, M. & Pozzi, G. (1992). Electron holography in the study of the electrostatic fields—The case of charged microtips. Ultramicroscopy 45(1), 77–83.10.1016/0304-3991(92)90039-MGoogle Scholar
Meyza, X., Goeuriot, D., Guerret-Piecourt, C., Treheux, D. & Fitting, H.J. (2003). Secondary electron emission and self-consistent charge transport and storage in bulk insulators: Application to alumina. J Appl Phys 94(8), 5384–5392.10.1063/1.1613807Google Scholar
Munger, B.L. (1977). The problem of specimen conductivity in electron microscopy. In Scanning Electron Microscopy I, Johari, O. (Ed.), pp. 481–490. Chicago: IITRI.Google Scholar
Ohno, S., Hora, K., Furukawa, T. & Oguchi, H. (1992). Ultrastructural-study of the glomerular slit diaphragm in fresh unfixed kidneys by a quick-freezing method. Virchows Arch B Cell Pathol Incl Mol Pathol 61(5), 351–358.10.1007/BF02890438Google Scholar
Okada, H., Shindo, D., Kim, J.J., Murakami, Y. & Kawase, H. (2007). Triboelectricity evaluation of single toner particle by electron holography. J Appl Phys 102, 054908/1–5.10.1063/1.2777566Google Scholar
Rodnyansky, A., Warburton, Y.J. & Hanke, L.D. (2000). Segregation in hot-dipped galvanized steel. Surf Interface Anal 29(3), 215–220.10.1002/(SICI)1096-9918(200003)29:3<215::AID-SIA703>3.0.CO;2-X3.0.CO;2-X>Google Scholar
Shi, X., Briseno, A.L., Sanedrin, R.J. & Zhou, F. (2003). Formation of uniform polyaniline thin shells and hollow capsules using polyelectrolyte-coated microspheres as templates. Macromolecules 36, 4093–4098.10.1021/ma034185gGoogle Scholar
Shindo, D., Kim, J.J., Kim, K.H., Xia, W.X., Ohno, N., Fujii, Y., Terada, N. & Ohno, S. (2009). Determination of orbital location of electron-induced secondary electrons by electric field visualization. J Phys Soc Jpn 78(10), 104802/1–8.10.1143/JPSJ.78.104802Google Scholar
Shindo, D., Kim, J.J., Xia, W.X., Kim, K.H., Ohno, N., Fujii, Y., Terada, N. & Ohno, S. (2007). Electron holography on dynamic motion of secondary electrons around sciatic nerve tissues. J Electron Microsc 56(1), 1–5.10.1093/jmicro/dfl039Google Scholar
Shindo, D. & Oikawa, T. (2002). Analytical Electron Microscopy for Materials Science. Tokyo: Springer-Verlag.10.1007/978-4-431-66988-3Google Scholar
Shindo, D., Park, Y.G., Murakami, Y., Gao, Y., Kanekiyo, H. & Hirosawa, S. (2003). Electron holography of Nd-Fe-B nanocomposite magnets. Scr Mater 48(7), 851–856.10.1016/S1359-6462(02)00601-2Google Scholar
Touzin, M., Goeuriot, D., Guerret-Piecourt, C., Juve, D., Treheux, D. & Fitting, H.J. (2006). Electron beam charging of insulators: A self-consistent flight-drift model. J Appl Phys 99(11), 114110/1–14.10.1063/1.2201851Google Scholar
Wepf, R., Amrein, M., Burkli, U. & Gross, H. (1991). Platinum iridium carbon—a high-resolution shadowing material for TEM, STM and SEM of biological macromolecular structures. J Microsc 163, 51–64.10.1111/j.1365-2818.1991.tb03159.xGoogle Scholar