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Effects of Natural Radiation, Photosynthetically Active Radiation and Artificial Ultraviolet Radiation-B on the Chloroplast Organization and Metabolism of Porphyra acanthophora var. brasiliensis (Rhodophyta, Bangiales)

Published online by Cambridge University Press:  16 November 2012

Zenilda L. Bouzon ,
Fungyi Chow ,
Carmen S. Zitta ,
Rodrigo W. dos Santos ,
Luciane C. Ouriques ,
Marthiellen R. de L. Felix ,
Luz K.P. Osorio ,
Claudiane Gouveia ,
Roberta de Paula Martins  and
Alexandra Latini
...Show all authors
Zenilda L. Bouzon
Affiliation:
Central Laboratory of Electron Microscopy, Federal University of Santa Catarina88049-900, CP 476, Florianópolis, SC, Brazil
Fungyi Chow
Affiliation:
Institute of Bioscience, University of São Paulo05508-090, São Paulo, SP, Brazil
Carmen S. Zitta
Affiliation:
Post-Graduate Program in Cell Biology and Development, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina88049-900, CP 476, Florianópolis, SC, Brazil
Rodrigo W. dos Santos
Affiliation:
Post-Graduate Program in Cell Biology and Development, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina88049-900, CP 476, Florianópolis, SC, Brazil
Luciane C. Ouriques
Affiliation:
Post-Graduate Program in Cell Biology and Development, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina88049-900, CP 476, Florianópolis, SC, Brazil
Marthiellen R. de L. Felix
Affiliation:
Department of Botany, Federal University of Santa Catarina88010-970, CP 476, Florianópolis, SC, Brazil
Luz K.P. Osorio
Affiliation:
Department of Botany, Federal University of Santa Catarina88010-970, CP 476, Florianópolis, SC, Brazil
Claudiane Gouveia
Affiliation:
Department of Botany, Federal University of Santa Catarina88010-970, CP 476, Florianópolis, SC, Brazil
Roberta de Paula Martins
Affiliation:
Laboratory of Bioenergetic and Oxidative Stress, Department of Biochemistry, Federal University of Santa Catarina88049-900, CP 476, Florianópolis, SC, Brazil
Alexandra Latini
Affiliation:
Laboratory of Bioenergetic and Oxidative Stress, Department of Biochemistry, Federal University of Santa Catarina88049-900, CP 476, Florianópolis, SC, Brazil
Fernanda Ramlov
Affiliation:
Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina88049-900, CP 476, Florianópolis, SC, Brazil
Marcelo Maraschin
Affiliation:
Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina88049-900, CP 476, Florianópolis, SC, Brazil
Eder C. Schmidt*
Affiliation:
Post-Graduate Program in Cell Biology and Development, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina88049-900, CP 476, Florianópolis, SC, Brazil
*
*Corresponding author. E-mail: [email protected]
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Abstract

We undertook a study of Porphyra acanthophora var. brasiliensis to determine its responses under ambient conditions, photosynthetically active radiation (PAR), and PAR+UVBR (ultraviolet radiation-B) treatment, focusing on changes in ultrastructure, and cytochemistry. Accordingly, control ambient samples were collected in the field, and two different treatments were performed in the laboratory. Plants were exposed to PAR at 60 μmol photons m−2 s−1 and PAR + UVBR at 0.35 W m−2 for 3 h per day during 21 days of in vitro cultivation. Confocal laser scanning microscopy analysis of the vegetative cells showed single stellate chloroplast in ambient and PAR samples, but in PAR+UVBR-exposed plants, the chloroplast showed alterations in the number and form of arms. Under PAR+UVBR treatment, the thylakoids of the chloroplasts were disrupted, and an increase in the number of plastoglobuli was observed, in addition to mitochondria, which appeared with irregular, disrupted morphology compared to ambient and PAR samples. After UVBR exposure, the formation of carpospores was also observed. Plants under ambient conditions, as well as those treated with PAR and PAR+UVBR, all showed different concentrations of enzymatic response, including glutathione peroxidase and reductase activity. In summary, the present study demonstrates that P. acanthophora var. brasiliensis shows the activation of distinct mechanisms against natural radiation, PAR and PAR+UVBR.

Keywords

carposporeschloroplastsenzymatic responsephotosynthetic pigmentsPorphyra acanthophora var. brasiliensisultraviolet radiation-B
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 18 , Issue 6 , December 2012 , pp. 1467 - 1479
Copyright
Copyright © Microscopy Society of America 2012

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Footnotes

†

Zenilda L. Bouzon and Eder C. Schmidt should be considered as first authors.

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