This supplement is a continuing step in the development of Microscopy and Microanalysis. The journal was launched in 1995 under the title JMSA, as the official journal of the Microscopy Society of America. In 1997 the journal was renamed and placed in the hands of Springer-Verlag, one of the world's leading publishers of scientific journals. In order to ensure the completeness and integrity of library collections, the scientific articles from Volumes 1 and 2 of JMSA are reprinted here as a supplement to Microscopy and Microanalysis.