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Developing and Applying a Correlative Light and Electron Microscopy Technique to Overcome Inherent Transmission Electron Microscopy Shortcomings.

Published online by Cambridge University Press:  30 July 2021

Jonathan Franks ,
Mike Calderone ,
Natasha Erdman ,
Alan Watson  and
Simon Watkins
Jonathan Franks
Affiliation:
Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
Mike Calderone
Affiliation:
Center for Biologic Imaging, University of Pittsburgh, United States
Natasha Erdman
Affiliation:
JEOL USA Inc, Peabody, Massachusetts, United States
Alan Watson
Affiliation:
Center for Biologic Imaging, University of Pittsburgh, United States
Simon Watkins
Affiliation:
Center for Biological Imaging, University of Pittsburgh, United States

Abstract

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Type
Challenges and Advances in Electron Microscopy Research and Diagnosis of Diseases in Humans, Plants and Animals (FIG associated)
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 1398 - 1400
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Zhang, P. (2013). Correlative cryo-electron tomography and optical microscopy of cells. Current Opinion in Structural Biology, 23, 763-770. doi: 10.1016/j.sbi.2013.07.017CrossRefGoogle ScholarPubMed
Kaplan, M, Nicolas, WJ, Zhao, W, Carter, SD, Metskas, LA, Chreifi, G, Ghosal, D, Jensen, GJ. In Situ Imaging and Structure Determination of Biomolecular Complexes Using Electron Cryo-Tomography. Methods Mol Biol. 2021;2215:83-111. doi: 10.1007/978-1-0716-0966-8_4. PMID: 33368000.CrossRefGoogle ScholarPubMed
Franks, J, Wallace, CT, Shibata, M, Suga, M, Erdman, N, Stolz, DB, Watkins, SC. Correlative Fluorescence and Electron Microscopy in 3D-Scanning Electron Microscope Perspective. Curr Protoc Cytom. 2017 Apr 3;80:12.45.1-12.45.15. doi: 10.1002/cpcy.18. PMID: 28369763.Google ScholarPubMed