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Detection of Calcium in the Adhesive Material Obtained from the Plant Pathogen Colletotrichum Graminicola'. X Ray Microanalysis (Eds) Evidences

Published online by Cambridge University Press:  02 July 2020

B. Leite ,
M.L. Ishida ,
E. Alves ,
S.F. Pascholati  and
J.A. Sugui
B. Leite
Affiliation:
Department of Entomology, Plant Pathology and Agriculture Zoology, University of Sao Paulo, Brazil
M.L. Ishida
Affiliation:
Department of Entomology, Plant Pathology and Agriculture Zoology, University of Sao Paulo, Brazil
E. Alves
Affiliation:
Department of Entomology, Plant Pathology and Agriculture Zoology, University of Sao Paulo, Brazil
S.F. Pascholati
Affiliation:
Department of Entomology, Plant Pathology and Agriculture Zoology, University of Sao Paulo, Brazil
J.A. Sugui
Affiliation:
Department of Biochemistry, Federal University of Parana, Brazil
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Extract

The appressoria formation of Colletotrichum graminicola was monitored by scanning electron microscopy (SEM) coupled with an X Ray microanalysis system (EDS - Oxford Instrument LINK ISIS). Recently formed appressoria, an infection structure of plant pathogenic fungi, firmly glues itself to an artificial surface (polystyrene) as a consequence of the production of an adhesive material (AM). The nature of this material was already demonstrated to be mainly constituted of a glycoprotein (Sugui et al, PMPP, 1998). The objective of this work was to verify the involvement of divalent ions, specially calcium, in the process as whole.

The AM was isolated and purified from conidia that germinated on polystyrene Petri dishes. The primary AM was dialyzed against three liters of distilled water before being lyophilized. Subsequently, the material was placed on top of a carbon tape and observed by scanning electron microscopy under high vacuum (Fig. 1 and 2A). On the other hand, the same material was submitted to X Ray microanalysis without coating.

Type
Light and Electron Microscopic Techniques for the Study of Plant Pathogenic Fungi and Their Interactions with Host Plants
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 698 - 699
Copyright
Copyright © Microscopy Society of America

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References

References:

1Sugui, J. A. et al.,. Partial characterization of the extracelular matrix released onto hydrophobic surfaces by conidia and conidial germlings of Colletotrichum graminicola. Physiological and Molecular Plant Pathology 52 (1998) 411–425.CrossRefGoogle Scholar
2Donaldson, S. P. and Deacon, J. W.. Role of calcium in adhesion and germination of zoospores cysts of Pythium: a model to explain infection of host plants. Journal of General Microbiology 138 (1992)2051–2059.CrossRefGoogle Scholar