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Detection and Identification of Viruses in Economically Important Insects

Published online by Cambridge University Press:  02 July 2020

E. L. Styer  and
J. J. Hamm
E. L. Styer
Affiliation:
Veterinary Diagnostic and Investigational Lab, The University of Georgia, P. O. Box 1389, Tifton, GA31793
J. J. Hamm
Affiliation:
Insect Biology and Pest Management Research Laboratory, USDA-ARS, P. O. Box 748, Tifton, GA31793
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Extract

Economically important insects include pests of plants, animals and stored products as well as insects produced commercially (honey bees, silkworms, insects for fish bait and food for birds and zoo animals). Other insects are produced in large numbers for experimental purposes, biological control of insect pests and weeds and the production of sterile insects for population suppression. Insect viruses may affect morphology, physiology and behavior, often reducing longevity and reproductive potential. Thus insect viruses can be used as biological control agents of pest insects. Insect viruses may also interfere with the production or function of biological control agents (e.g., parasitoids and predators) and insects used for research purposes. Therefore, it is advantageous to screen commercial and research colonies and imported insects for viruses.

Electron microscopy of negatively stained specimens (NS EM) offers a relatively rapid and inexpensive means of screening populations of insects for the presence of viruses or viruslike particles and to monitor the progress of virus control programs.

Type
Microorganisms: The Good, The Bad, The Unusual
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 666 - 667
Copyright
Copyright © Microscopy Society of America

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References

References:

1.Doane, F. W. and Anderson, N., Electron Microscopy in Diagnostic Virology, Cambridge University Press (1987)Google ScholarPubMed
2.Evans, H. and Shapiro, M.., in Lacey, L., Ed., Manual of Techniques in Insect Pathology, San DiegoAcademic Press (1997) 17.CrossRefGoogle Scholar
3.Hamm, J. J. et al., Biological Control 2 (1992) 329.CrossRefGoogle Scholar
4. Among those who have submitted specimens for diagnostic purposes are: A. Liggett, VDIL, Tifton, GA; J. Carpenter, J. Lewis, D. Nordlund, G. Tillman, USDA/ARS, Tifton, GA; T. Coudron, W. Steiner, USDA/ARS, Columbia, MO; J. Lopez, USDA/ARS, College Station, TX; J. Molina, University of Colima, Colima, MX.Google Scholar