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Defective Histone H3K27 Trimethylation Modification in Embryos Derived from Heated Mouse Sperm

Published online by Cambridge University Press:  09 May 2012

Shi-Bin Chao ,
Lei Chen ,
Jian-Chun Li ,
Xiang-Hong Ou ,
Xiao-Jin Huang ,
Shu Wen ,
Qing-Yuan Sun  and
Guo-Lan Gao
Shi-Bin Chao
Affiliation:
Center of Reproductive Medicine, The First Affiliated Hospital of NanChang University, NanChang, China State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Lei Chen
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Jian-Chun Li
Affiliation:
Center of Reproductive Medicine, The First Affiliated Hospital of NanChang University, NanChang, China
Xiang-Hong Ou
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Xiao-Jin Huang
Affiliation:
Center of Reproductive Medicine, The First Affiliated Hospital of NanChang University, NanChang, China
Shu Wen
Affiliation:
Department of Obstetrics and Gynecology, One Baylor Plaza, Baylor College of Medicine, Houston, TX, USA
Qing-Yuan Sun
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Guo-Lan Gao*
Affiliation:
Center of Reproductive Medicine, The First Affiliated Hospital of NanChang University, NanChang, China Department of Obstetrics and Gynecology, Aviation General Hospital of China, Beijing, China
*
Corresponding author. E-mail: [email protected]
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Abstract

The mouse sperm genome is resistant to in vitro heat treatment, and embryos derived from heated sperm can support full-term embryonic development, but the blastocyst rate and implantation rate are lower compared to embryos derived from fresh sperm. In the present study, the patterns of DNA methylation, histone H4K12 (ACH4K12) acetylation, H3K9 trimethylation (H3K9-TriM), and H3K27 trimethylation (H3K27-TriM) in preimplantation embryos derived from 65°C-heated sperm were investigated. Although no evident changes in global DNA methyaltion, histone H4K12 (ACH4K12) acetylation, and H3K9 trimethylation (H3K9-TriM) were found, significantly lower levels of H3K27-TriM, which was thought to be one of the reasons for low efficiency of mouse cloning, were found in the inner cell mass of heated-sperm derived blastocysts. Thus, defective modification of H3K27-TriM might contribute to compromised development of embryos derived from heated sperm.

Keywords

intracytoplasmic sperm injection (ICSI)epigenetic reprogrammingDNA methylationhistone modificationinner cell mass (ICM)
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 18 , Issue 3 , June 2012 , pp. 476 - 482
Copyright
Copyright © Microscopy Society of America 2012

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