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Customizable Cryo-EM Chips Improve 3D Analysis of Macromolecules

Published online by Cambridge University Press:  05 August 2019

A. Cameron Varano ,
Nick Alden ,
William Dearnaley ,
Michael Casasanta ,
John Damiano ,
Jennifer McConnell ,
Madeline Dukes  and
Deborah F. Kelly
A. Cameron Varano*
Affiliation:
Virginia Tech Carilion Research Institute, Virginia Tech, Roanoke, VA, USA. Translational Biology, Medicine and Health, Virginia Tech, Blacksburg, VA, USA. Department of Biomedical Engineering, Pennsylvania State University, University Park, PA, USA. Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA. Center for Structural Onocology, Pennsylvania State University, University Park, PA, USA.
Nick Alden
Affiliation:
Virginia Tech Carilion Research Institute, Virginia Tech, Roanoke, VA, USA.
William Dearnaley
Affiliation:
Virginia Tech Carilion Research Institute, Virginia Tech, Roanoke, VA, USA. Department of Biomedical Engineering, Pennsylvania State University, University Park, PA, USA. Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA. Center for Structural Onocology, Pennsylvania State University, University Park, PA, USA.
Michael Casasanta
Affiliation:
Department of Biomedical Engineering, Pennsylvania State University, University Park, PA, USA. Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA. Center for Structural Onocology, Pennsylvania State University, University Park, PA, USA.
John Damiano
Affiliation:
Application Science, Protochips Inc., Morrisville, NC, USA.
Jennifer McConnell
Affiliation:
Application Science, Protochips Inc., Morrisville, NC, USA.
Madeline Dukes
Affiliation:
Application Science, Protochips Inc., Morrisville, NC, USA.
Deborah F. Kelly
Affiliation:
Virginia Tech Carilion Research Institute, Virginia Tech, Roanoke, VA, USA. Department of Biomedical Engineering, Pennsylvania State University, University Park, PA, USA. Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA. Center for Structural Onocology, Pennsylvania State University, University Park, PA, USA.
*
*Correspondence to: [email protected]

Abstract

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Type
3D Structures: from Macromolecular Assemblies to Whole Cells (3DEM FIG)
Information
Microscopy and Microanalysis , Volume 25 , Supplement S2 , August 2019 , pp. 1310 - 1311
Copyright
Copyright © Microscopy Society of America 2019 

References

[1]Scheres, S. H., Journal of Structural Biology 180 (2012), p. 519.Google Scholar
[2]Glaeser, R. M., Nature Methods 13 (2016), p. 28.Google Scholar
[3]Winton, C. E. et al. , Microscopy Today 25(4) (2017). p. 22.Google Scholar
[4]The authors acknowledge the following funding sources that supported this work NIH/NCI R01CA193578, R01CA227261, R01CA219700.Google Scholar