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Current Applications and Methods of Microvascular Corrosion Casting: A Review

Published online by Cambridge University Press:  02 July 2020

A. Lametschwandtner ,
H. Aichhorn  and
B. Minnich
A. Lametschwandtner
Affiliation:
Department of Experimental Zoology, University of Salzburg, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria(Europe)
H. Aichhorn
Affiliation:
Department of Experimental Zoology, University of Salzburg, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria(Europe)
B. Minnich
Affiliation:
Department of Experimental Zoology, University of Salzburg, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria(Europe)
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Extract

Casting of hollow spaces with solidifying materials and subsequent removal of surrounding tissues by corrosive alkali and acids and the inspection of the remaining casts by bare eyes or the dissecting microscope is an old anatomical technique.

The introduction of polymerizing resins as casting materials which resulted in durable casts of even the smallest spaces (bile and blood capillaries) and the application of the scanning electron microscope with its high resolution and great depth of focus, enabled the qualitative and the quantitative analysis of the 3D-arrangement of tubular systems by means of their casts.

Presently, scanning electron microscopy of microvascular corrosion casts is used to study growing, stable or regressing blood vessel systems under physiological (e.g. during development, wound healing, metamorphosis) and pathological (e.g. tumor angiogenesis) conditions in qualitative and quantitative terms.

Type
Applications and Methods of Vascular Corrosion Casting—The 3-Dimensional Microvasculature of Tissues
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 908 - 909
Copyright
Copyright © Microscopy Society of America

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References

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