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Confocal Scanning Laser Microscopy, Scanning and Transmission Electron Microscopy of Vertebrate Cerebellar Granule Cells

Published online by Cambridge University Press:  02 July 2020

O. J. Castejón ,
H.V. Castejón  and
R. P. Apkarian
O. J. Castejón
Affiliation:
Instituto de Investigaciones Biológicas., Facultad de Medicina., Universidad del Zulia., Maracaibo.-, Venezuela
H.V. Castejón
Affiliation:
Instituto de Investigaciones Biológicas., Facultad de Medicina., Universidad del Zulia., Maracaibo.-, Venezuela
R. P. Apkarian
Affiliation:
Department of Chemistry., Emory University., Atlanta, GA.
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Abstract

The cerebellar granule cells of hamsters, mice, primates and human have been examined to study their organisation, outer and inner surfaces, pattern of connectivity on granule cell groups and synaptic relationships with afferent mossy and climbing fibers in the granule cell layer and with Purkinje cell dendritic spines in the molecular layer.

Confocal laser scanning microscopy of hamster cerebellum cortex, using FM4-64 as an intracellular stain, showed in z-series of stacks of optodigital sections, the presence of granule cell groups formed by small, medium and large granule cells, ranging from 3-10 μm in diameter, surrounded by large Golgi cells. The granule cell/Golgi cell ratio was 50 to 4 (Fig. 1).

Cryofractured mouse cerebellar cortex showed the inner organization of granule cell nuclear and cytoplasmic compartments in samples examined with FEURSEM and coated with chromium (Fig. 2), which were compared with corresponding freeze-etching replica images.

Type
Neurobiology
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 662 - 663
Copyright
Copyright © Microscopy Society of America 2001

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References

1.Castejon, O. J. and Castejon, H.V.. Conventional and high resolution scanning electron microscopy of cerebellar Purkinje cells. Biocell 21(1997)149–159.Google Scholar
2.Castejon, O. J. and Castejon, H.V.. Correlative microscopy of cerebellar Golgi cells. Biocell 24(2000) 13–30.Google ScholarPubMed
3.Castejon, O. J. et al. Confocal, scanning and transmision electron microscopic study of cerebellar mossy fiber glomeruli. J. Submicrosc. Cytol. Pathol. 32 (2000) 247–260.Google Scholar
4.This research was partially supported by CONDES-LUZ, Department of Chemistry, Emory University and IMR Wisconsin University, Madison.Google Scholar