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Confocal Microscopy, Computer Modeling, and Quantification of Glomerular Vascular Corrosion Casts

Published online by Cambridge University Press:  16 May 2006

Roger C. Wagner ,
Kirk Czymmek  and
Fred E. Hossler
Roger C. Wagner
Affiliation:
Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
Kirk Czymmek
Affiliation:
Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA
Fred E. Hossler
Affiliation:
Department of Anatomy and Cell Biology, J.H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614, USA
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Abstract

Corrosion-casted capillary systems of the kidney glomerulus were imaged with confocal microscopy because of the fluorescence properties of the casting plastic. Acquisition of a z-series through the glomerular capillaries provided three-dimensional data sets from which surface-rendered models were generated. These models could be rotated and viewed from any angle and also contained quantitative information allowing cast surface area and volume measurements to be calculated. The computer-generated models were also skeletonized to form a one-voxel-thick skeleton of the original model. The skeleton exhibited the three-dimensional topology and network of the capillary bed, and interior capillary relations could also be viewed. Quantitative information such as the total capillary length and number of capillary intersects was calculated from the skeletonized model. Extending this method to noncorroded kidney specimens revealed not only the casted vessels but also cellular features of the adjacent tissues surrounding the capillaries.

Keywords

kidneyglomeruluscorrosion castsconfocal microscopycomputer modelingskeletonization
Type
BIOLOGICAL APPLICATIONS
Information
Microscopy and Microanalysis , Volume 12 , Issue 3 , June 2006 , pp. 262 - 268
Copyright
© 2006 Microscopy Society of America

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