Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-26T13:37:56.991Z Has data issue: false hasContentIssue false

Confocal Fluorescence Microscopy with CCD Sensors Speed by Parallelization, Low Cost by Using More Electronics

Published online by Cambridge University Press:  02 July 2020

Rudolf E. Großkopf*
Affiliation:
ConflmagingResearch (CIR), E-mail: [email protected]
Get access

Extract

CCD sensors are able to image a few megapixels within one frame. The image is scanned electronically within the semiconductor chip (instead of mechanical-optical scanning with moving mirrors, Nipkow discs or acousto-optical devices). Through parallelization. the novel concept yields speed at an unprecedented degree for confocal imaging Routine applications requiring high speed and low costs will profit from this principle. Thus, confocal imaging technology will take the same path television technology has taken—from mechanical scanning to the broadest possible application of electronics

In order to go this way, a pinhole matrix is used on the illumination side. It has as many pinholes and the same pitch as the pitch and number of pixels of the CCD (Figure 1). In front of the receiver, a second pinhole matrix with the same pitch and number of pinholes is used All pinholes of both matrices and the pixels of the CCD are in confocal position.

Type
Confocal Microscopy
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

References:

1.Knupfer, K., Derndinger, E.. Großkopf, R., Vorrichtung zur dreidimensionalen Untersuchung eines Objektes. Deutsche Patentschrift DE 40 35799 (1995)Google Scholar
2.Schröck, E.. Spektrale Kariotypisierung und vergleichende genomische Hybridisierung—neue Methoden zur umfassenden Analyse chromosomaler Aberrationen in der klinisch-genetischen Diagnostik und der Tumorgenetik, post-doctoral thesis, Humboldt Universität in Berlin, 1998Google Scholar
3.Bosiers, J.T.. et al, A 2/3” 2-M pixel progressive scan FT-CCD for still camera applications, IEDM Techn. Dig., pp 3740. San Fancisco. Dec. 1998Google Scholar