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Computational Recovery of Engineered Point Spread Functions in Single Molecule Localization Microscopy using the Double Helix 3DTRAX Software

Published online by Cambridge University Press:  30 July 2021

Scott Gaumer
Affiliation:
Double Helix Optics, United States
Warren Colomb
Affiliation:
Double Helix Optics, United States
Anjul Loiacono
Affiliation:
Double Helix Optics, United States
Leslie Kimerling
Affiliation:
Double Helix Optics, Boulder, Colorado, United States
Anurag Agrawal
Affiliation:
Double Helix Optics, United States

Abstract

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Type
Frontiers in Fluorescence Lifetime and Super-resolution Imaging of Biological Structures and Dynamics
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Rust, QM. J., et al. , “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).,Nat. Methods, vol. 3, no. 10, pp. 793–5, Oct. 2006.CrossRefGoogle ScholarPubMed
Pavani, S. R. P. et al. , “High-efficiency rotating point spread functions,Opt. Express, vol. 16, no. 5, pp. 34843489, Mar. 2008.CrossRefGoogle Scholar
Grover, G., et al. , “Performance limits on three-dimensional particle localization in photon-limited microscopy,Opt. Lett., vol. 35, no. 19, p. 3306, Sep. 2010.Google Scholar
Shechtman, Y., et al. , “Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions,Nano Lett., vol. 15, no. 6, pp. 41944199, Jun. 2015.CrossRefGoogle Scholar
Jain, S., et al. , “ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure,Cell, vol. 164, no. 3, pp. 487498, 2016.Google Scholar
Wang, D., et al. , “Enhanced information content for three-dimensional localization and tracking using the double-helix point spread function with variable-angle illumination epifluorescence microscopy,Appl. Phys. Lett., vol. 110, no. 21, p. 211107, May 2017.CrossRefGoogle Scholar
Gahlmann, A. et al. , “Quantitative multicolor subdiffraction imaging of bacterial protein ultrastructures in three dimensions.,” Nano Lett., vol. 13, no. 3, pp. 987–93, Mar. 2013.CrossRefGoogle ScholarPubMed
Pavani, S. R. P. et al. , “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function.,” Proc. Natl. Acad. Sci. U. S. A., vol. 106, no. 9, pp. 2995–9, Mar. 2009.CrossRefGoogle ScholarPubMed
Gustavsson, A.-K., et al. , “3D single-molecule super-resolution microscopy with a tilted light sheet,Nat. Commun. 2018 91, vol. 9, no. 1, p. 123, Jan. 2018.Google Scholar
Shechtman, Y., et al. , “Multicolour localization microscopy by point-spread-function engineering,Nat. Photonics, vol. 10, no. 9, pp. 590594, Sep. 2016.Google Scholar
Lam, W. Y. et al. , “HER2 Cancer Protrusion Growth Signaling Regulated by Unhindered, Localized Filopodial Dynamics,bioRxiv, p. 654988, Jun. 2019.Google Scholar