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Colocalization Analysis of PPP1 Isoforms and Two Novel Targeting Subunits in Breast Carcinoma

Published online by Cambridge University Press:  03 October 2008

C. Sousa ,
A.P. Vintém ,
M. Fardilha ,
O. da Cruz e Silva  and
E. da Cruz e Silva
C. Sousa*
Affiliation:
Signal Transduction Lab, CBC and Department of Biology, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal
A.P. Vintém
Affiliation:
Signal Transduction Lab, CBC and Department of Biology, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal
M. Fardilha
Affiliation:
Signal Transduction Lab, CBC and Department of Biology, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal
O. da Cruz e Silva
Affiliation:
Neurosciences Lab, CBC, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal
E. da Cruz e Silva
Affiliation:
Signal Transduction Lab, CBC and Department of Biology, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal
*
Corresponding author.

Abstract

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Protein phosphatase 1 (PPP1) is the PPP most ubiquitous and each isoform interact with regulatory subunits that may be responsible for their subcellular localization. We identified PPP1R15B, C1ORF71 as novel regulators and the aim of this study was their further characterization in carcinoma cells. We analysed localization of each regulator in MDA-MB-468 cells and we transfected with constructs that we made with each as a GFP-fusion protein. For PPP1 cellular localization we used specific antibodies for each isoform. We observed the cells under a fluorescent microscope and LSM and we quantified co-localization. We found a high overlap coefficient of both the novel proteins with PPP1alpha and PPP1gamma1. We propose a model in which PPP1 regulator interacts with one or two regulatory subunits that may be used as target for therapeutic strategies.

Keywords

fluorescent microscopylaser scanning microscopyoverlap coefficientPPP1novel regulatorsbreast carcinoma
Type
Abstract
Information
Microscopy and Microanalysis , Volume 14 , Supplement S3 , September 2008 , pp. 134 - 136
Copyright
Copyright © Microscopy Society of America 2008