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Colloidal Palladium Particles of Different Shapes for Electron Microscopy Labeling

Published online by Cambridge University Press:  24 December 2009

Daryl A. Meyer ,
Julie A. Oliver  and
Ralph M. Albrecht
Daryl A. Meyer
Affiliation:
Department of Animal Sciences, University of Wisconsin, 1675 Observatory Dr., Madison, WI 53706-1284, USA
Julie A. Oliver
Affiliation:
Department of Animal Sciences, University of Wisconsin, 1675 Observatory Dr., Madison, WI 53706-1284, USA
Ralph M. Albrecht*
Affiliation:
Department of Animal Sciences, University of Wisconsin, 1675 Observatory Dr., Madison, WI 53706-1284, USA
*
Corresponding author. E-mail: [email protected]
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Abstract

The immunogold technique is a valuable method for labeling cellular macromolecules. However, multiple labeling using colloidal gold (cAu) nanoparticles of different sizes presents certain drawbacks; namely, as particle size increases, there is a decreased labeling efficiency and diminished spatial resolution with respect to the locations of labeled epitopes. Both concerns also limit the utility of heavy metal particles for comparative analysis of labeling densities. To minimize the variables due to differential labeling efficiencies, the best solution would be to conduct multiple labeling with particles of similar size. Consequently, some parameter other than size is necessary to distinguish each label type. In this study, we report the synthesis of colloidal palladium (cPd) nanoparticles of similar size but having two distinct shapes, umbonate and faceted, which are readily distinguishable from spherical colloidal gold particles. Their utility and fidelity as labels using a human platelet whole-mount model is also demonstrated.

Keywords

electron microscopy (EM)labelsshapescolloidal palladiumnanoparticlescolloidal goldplatelets
Type
Biological Imaging: Techniques Development and Applications
Information
Microscopy and Microanalysis , Volume 16 , Issue 1 , February 2010 , pp. 33 - 42
Copyright
Copyright © Microscopy Society of America 2010

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