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Characterization of Structural Changes of Casein Micelles at Different pH Using Microscopy and Spectroscopy Techniques

Published online by Cambridge University Press:  14 February 2022

Liliana Edith Rojas-Candelas ,
José Jorge Chanona-Pérez Open the ORCID record for José Jorge Chanona-Pérez [Opens in a new window] ,
Juan Vicente Méndez Méndez ,
José Antonio Morales-Hernández  and
Héctor Alfredo Calderón Benavides
Liliana Edith Rojas-Candelas
Affiliation:
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu Esq, Cda, Miguel Stampa s/n, C.P. 07738 Mexico City, Mexico
José Jorge Chanona-Pérez*
Affiliation:
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu Esq, Cda, Miguel Stampa s/n, C.P. 07738 Mexico City, Mexico
Juan Vicente Méndez Méndez
Affiliation:
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu Esq, Cda, Miguel Stampa s/n, C.P. 07738 Mexico City, Mexico Centro de Nanociencias y Micro y Nanotecnologías, Instituto Politécnico Nacional, Luis Enrique Erro s/n, Zacatenco, Gustavo A. Madero, C.P. 07738 Mexico City, Mexico
José Antonio Morales-Hernández
Affiliation:
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), A. C. Av Normalistas #800, Guadalajara, Jalisco, México
Héctor Alfredo Calderón Benavides
Affiliation:
Instituto Politécnico Nacional. Escuela Superior de Física y Matemáticas, Av. Instituto Politécnico Nacional Edificio 9, U. Profesional Adolfo Lopez Mateos, Gustavo A. Madero, C.P. 07738 Mexico City, Mexico
*
*Corresponding author: José Jorge Chanona-Pérez E-mail: [email protected]
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Abstract

This study aimed to evaluate the influence of pH changes on morphometric parameters of casein micelles and a general overview of their conformational structure through microscopy techniques, Raman spectroscopy and multivariate analysis. It was found that casein micelles morphology and protein secondary structure depend strongly upon pH. The changes of arithmetic average roughness (Ra), size, and shape of casein micelles at different pH are properly characterized by atomic force and cryo-transmission electron microscopy. Morphometric changes of casein micelles were correlated correctly with folding and unfolding of casein molecules as evaluated by Raman spectroscopy when the pH was varied. The novelty of this contribution consists in demonstrating that there is a close structure-functionality relationship between the morphometric parameters of proteins and their secondary structure. Knowledge about casein micelles can help improve their use of its diverse applications.

Keywords

atomic force microscopycasein micelle structurecryo-transmission electron microscopymultivariate analysisRaman spectroscopy
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 28 , Issue 2 , April 2022 , pp. 527 - 536
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of the Microscopy Society of America

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