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Cardiomyocyte Apoptosis in the Outflow Tract in Normal and Abnormal Cardiogenesis

Published online by Cambridge University Press:  02 July 2020

Michiko Watanabe ,
Midori Hitomi ,
Denver Sallee ,
Anjum Jafri ,
Florence Rothenberg ,
Kathy van der Wee  and
Steven A. Fisher
Michiko Watanabe
Affiliation:
Departments of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH, 44106.
Midori Hitomi
Affiliation:
Departments of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH, 44106.
Denver Sallee
Affiliation:
Departments of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH, 44106.
Anjum Jafri
Affiliation:
Medicine, Case Western Reserve University School of Medicine, Cleveland, OH, 44106.
Florence Rothenberg
Affiliation:
Medicine, Case Western Reserve University School of Medicine, Cleveland, OH, 44106.
Kathy van der Wee
Affiliation:
Medicine, Case Western Reserve University School of Medicine, Cleveland, OH, 44106.
Steven A. Fisher
Affiliation:
Medicine, Case Western Reserve University School of Medicine, Cleveland, OH, 44106.
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Abstract

Apoptosis peaks within the cardiac outflow tract (OFT) myocardium during chick development (stage 30-31) and coincides with dramatic remodeling of the OFT 4. Apoptosis was assayed by TUNEL, Annexin V, transmission electron microscopy, and Lysotracker Red (Fig. 1-4). These assays reveal that as many as 92% of the apoptotic cells express cardiomyocyte markers. The timing of apoptosis suggests that it may occur to allow proper alignment of the great vessels over their respective ventricles. Our current aims are to determine the consequences of disturbing OFT cardiomyocyte cell death to heart morphogenesis and to elucidate the cellular and molecular components that initiate and regulate this high level of apoptosis.

To enhance apoptosis in the OFT myocardium, recombinant adenoviruses were injected into the pericardial space to deliver the gene for the death ligand Fas-L to the cardiomyocytes of chicken embryos prior to the stages when OFT remodeling occurs.

Type
Apoptosis in Health and Disease: Techniques for Detection and Biological Importance (Organized by M. Watanabe)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 594 - 595
Copyright
Copyright © Microscopy Society of America 2001

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References

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5. Supported by AHA Ohio Valley Fellowship (FR), AHA GIA, NIH (SAF), NIH, NSF (MW). Authors thank Dr. K. Walsh for the recombinant adenoviruses and Dr. Kirby and colleagues for their generous advice and contribution of the cardiac neural crest ablated chicken embryos.