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Bond Strength and Bioactivity of Zn-Doped Dental Adhesives Promoted by Load Cycling

Published online by Cambridge University Press:  11 December 2014

Manuel Toledano ,
Fátima S. Aguilera ,
Estrella Osorio ,
Inmaculada Cabello ,
Manuel Toledano-Osorio  and
Raquel Osorio
Manuel Toledano*
Affiliation:
Faculty of Dentistry, Dental Materials Section, University of Granada, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain
Fátima S. Aguilera
Affiliation:
Faculty of Dentistry, Dental Materials Section, University of Granada, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain
Estrella Osorio
Affiliation:
Faculty of Dentistry, Dental Materials Section, University of Granada, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain
Inmaculada Cabello
Affiliation:
Faculty of Dentistry, Dental Materials Section, University of Granada, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain
Manuel Toledano-Osorio
Affiliation:
Faculty of Dentistry, Dental Materials Section, University of Granada, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain
Raquel Osorio
Affiliation:
Faculty of Dentistry, Dental Materials Section, University of Granada, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain
*
*Corresponding author.[email protected]
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Abstract

The purpose of this study was to evaluate if mechanical loading influences bioactivity and bond strength at the resin–dentin interface after bonding with Zn-doped etch-and-rinse adhesives. Dentin surfaces were subjected to demineralization by 37% phosphoric acid (PA) or 0.5 M ethylenediaminetetraacetic acid (EDTA). Single bond (SB) adhesive—3M ESPE—SB+ZnO particles 20 wt% and SB+ZnCl2 2 wt% were applied on treated dentin to create the groups PA+SB, SB+ZnO, SB+ZnCl2, EDTA+SB, EDTA+ZnO, and EDTA+ZnCl2. Bonded interfaces were stored in simulated body fluid for 24 h and tested or submitted to mechanical loading. Microtensile bond strength (MTBS) was assessed. Debonded dentin surfaces were studied by high-resolution scanning electron microscopy. Remineralization of the bonded interfaces was assessed by atomic force microscope imaging/nanoindentation, Raman spectroscopy/cluster analysis, and Masson’s trichrome staining. Load cycling (LC) produced reduction in MTBS in all PA+SB, and no change was encountered in EDTA+SB specimens, regardless of zinc doping. LC increased the mineralization and crystallographic maturity at the interface; a higher effect was noticed when using ZnO. Trichrome staining reflected a narrow demineralized dentin matrix after loading of dentin surfaces that were treated with SB-doped adhesives. This correlates with an increase in mineral platforms or plate-like multilayered crystals in PA or EDTA-treated dentin surfaces, respectively.

Keywords

dentinremineralizationload cyclingdoped adhesivesbond strength
Type
Biological and Biomaterials Applications
Information
Microscopy and Microanalysis , Volume 21 , Issue 1 , February 2015 , pp. 214 - 230
Copyright
© Microscopy Society of America 2014 

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References

Ager, J.W., Nalla, R.K., Breeden, K.L. & Ritchie, R.O. (2005). Deep-ultraviolet Raman spectroscopy study of the effect of aging on human cortical bone. J Biomed Opt 10, 034012.CrossRefGoogle ScholarPubMed
Almahdy, A., Downey, F.C., Sauro, S., Cook, R.J., Sherriff, M., Richards, D., Watson, T.F., Banerjee, A. & Festy, F. (2012). Microbiochemical analysis of carious dentin using Raman and fluorescence spectroscopy. Caries Res 46, 432–440.CrossRefGoogle ScholarPubMed
Awonusi, A., Morris, M.D. & Tecklenburg, M.M. (2007). Carbonate assignment and calibration in the Raman spectrum of apatite. Calcif Tissue Int 81, 46–52.CrossRefGoogle ScholarPubMed
Balooch, M., Habelitz, S., Kinney, J.H., Marshall, S.J. & Marshall, G.W. (2008). Mechanical properties of mineralized collagen fibrils as influenced by demineralization. J Struct Biol 162, 404–410.CrossRefGoogle ScholarPubMed
Daood, U., Iqbal, K., Nitisusanta, L.I. & Fawzy, A.S. (2013). Effect of chitosan/riboflavin modification on resin/dentin interface: Spectroscopic and microscopic investigations. J Biomed Mater Res A 101, 1846–1856.CrossRefGoogle ScholarPubMed
De Munck, J., Van Meerbeek, B., Yoshida, Y., Inoue, S., Vargas, M., Suzuki, K., Lambrechts, P. & Vanherle, G. (2003). Four-year water degradation of total-etch adhesives bonded to dentin. J Dent Res 82, 136–140.CrossRefGoogle ScholarPubMed
Erhardt, M.C., Osorio, R. & Toledano, M. (2008). Dentin treatment with MMPs inhibitors does not alter bond strengths to caries-affected dentin. J Dent 36, 1068–1073.CrossRefGoogle Scholar
Gandolfi, M.G., Taddei, P., Siboni, F., Modena, E., De Stefano, E.D., Prati, C. 2011). Biomimetic remineralization of human dentin using promising innovative calcium-silicate hybrid “smart” materials. Dent Mater 27, 1055–1069.CrossRefGoogle ScholarPubMed
Habelitz, S., Balooch, M., Marshall, S.J., Balooch, G. & Marshall, G.W. Jr. (2002). In situ atomic force microscopy of partially demineralized human dentin collagen fibrils. J Struct Biol 138, 227–236.CrossRefGoogle ScholarPubMed
Jastrzebska, M., Wrzalik, R., Kocot, A., Zalewska-Rejdak, J. & Cwalina, B. (2003). Raman spectroscopic study of glutaraldehyde-stabilized collagen and pericardium tissue. J Biomater Sci Polym Ed 14, 185–197.CrossRefGoogle ScholarPubMed
Karan, K., Yao, X., Xu, C. & Wang, Y. (2009). Chemical profile of the dentin substrate in non-carious cervical lesions. Dent Mater 25, 1205–1212.CrossRefGoogle ScholarPubMed
Koibuchi, H., Yasuda, N. & Nakabayashi, N. (2001). Bonding to dentin with a self-etching primer: The effect of smear layers. Dent Mater 17, 122–126.CrossRefGoogle ScholarPubMed
Krajewski, A., Ravaglioli, A., Tinti, A., Taddei, P., Mazzocchi, M., Martinetti, R., Fagnano, C. & Fini, M. (2005). Comparison between the in vitro surface transformations of AP40 and RKKP bioactive glasses. J Mater Sci Mater Med 16, 119–128.CrossRefGoogle ScholarPubMed
Kremer, E.A., Chen, Y., Suzuki, K., Nagase, H. & Gorski, J.P. (1998). Hydroxyapatite induces autolytic degradation and inactivation of matrix metalloproteinase-1 and -3. J Bone Miner Res 13, 1890–1902.CrossRefGoogle ScholarPubMed
Marshall, G.W. Jr., Marshall, S.J., Kinney, J.H. & Balooch, M. (1997). The dentin substrate: Structure and properties related to bonding. J Dent 25, 441–458.CrossRefGoogle ScholarPubMed
Milly, H., Festy, F., Watson, T.F., Thompson, I. & Banerjee, A. (2014). Enamel white spot lesions can remineralise using bio-active glass and polyacrylic acid-modified bio-active glass powders. J Dent 42, 158–166.CrossRefGoogle ScholarPubMed
Nakabayashi, N. (1992). The hybrid layer: A resin-dentin composite. Proc Finn Dent Soc 88(Suppl 1), 321–329.Google Scholar
Nakabayashi, N., Kojima, K. & Masuhara, E. (1982). The promotion of adhesion by the infiltration of monomers into tooth substrates. J Biomed Mater Res 16, 265–273.CrossRefGoogle ScholarPubMed
Nikaido, T., Kunzelmann, K.H., Chen, H., Ogata, M., Harada, N., Yamaguchi, S., Cox, C.F., Hickel, R. & Tagami, J. (2002). Evaluation of thermal cycling and mechanical loading on bond strength of a self-etching primer system to dentin. Dent Mater 18, 269–275.CrossRefGoogle ScholarPubMed
Nudelman, F., Pieterse, K., George, A., Bomans, P.H., Friedrich, H., Brylka, L.J., Hilbers, P.A., De With, G. & Sommerdijk, N.A. (2010). The role of collagen in bone apatite formation in the presence of hydroxyapatite nucleation inhibitors. Nat Mater 9, 1004–1009.CrossRefGoogle ScholarPubMed
Osorio, R., Cabello, I. & Toledano, M. (2014). Bioactivity of zinc-doped dental adhesives. J Dent 42, 403–412.CrossRefGoogle ScholarPubMed
Osorio, R., Yamauti, M., Osorio, E., Ruiz-Requena, M.E., Pashley, D., Tay, F. & Toledano, M. (2011). Effect of dentin etching and chlorhexidine application on metalloproteinase-mediated collagen degradation. Eur J Oral Sci 119, 79–85.CrossRefGoogle ScholarPubMed
Pashley, D.H., Zhang, Y., Carvalho, R.M., Rueggeberg, F.A. & Russell, C.M. (2000). H+-induced tension development in demineralized dentin matrix. J Dent Res 79, 1579–1583.CrossRefGoogle ScholarPubMed
Prati, C., Chersoni, S. & Pashley, D.H. (1999). Effect of removal of surface collagen fibrils on resin-dentin bonding. Dent Mater 15, 323–331.CrossRefGoogle ScholarPubMed
Prati, C., Pashley, D.H., Chersoni, S. & Mongiorgi, R. (2000). Marginal hybrid layer in class V restorations. Oper Dent 25, 228–233.Google ScholarPubMed
Salehi, H., Terrer, E., Panayotov, I., Levallois, B., Jacquot, B., Tassery, H. & Cuisinier, F. (2013). Functional mapping of human sound and carious enamel and dentin with Raman spectroscopy. J Biophotonics 6, 765–774.CrossRefGoogle ScholarPubMed
Sauro, S., Osorio, R., Watson, T.F. & Toledano, M. (2012). Therapeutic effects of novel resin bonding systems containing bioactive glasses on mineral-depleted areas within the bonded-dentin interface. J Mater Sci Mater Med 23, 1521–1532.CrossRefGoogle Scholar
Schwartz, A.G., Pasteris, J.D., Genin, G.M., Daulton, T.L. & Thomopoulos, S. (2012). Mineral distributions at the developing tendon enthesis. PLoS One 7, e48630.CrossRefGoogle ScholarPubMed
Sell, D.R. & Monnier, V.M. (1989). Structure elucidation of a senescence cross-link from human extracellular matrix. Implication of pentoses in the aging process. J Biol Chem 264, 21597–21602.CrossRefGoogle ScholarPubMed
Toledano, M., Aguilera, F.S., Cabello, I. & Osorio, R. (2014 a). Remineralization of mechanical loaded resin-dentin interface: A transitional and synchronized multistep process. Biomech Model Mechanobiol 13, 1289–1302.CrossRefGoogle ScholarPubMed
Toledano, M., Aguilera, F.S., Sauro, S., Cabello, I., Osorio, E. & Osorio, R. (2014 b). Load cycling enhances bioactivity at the resin-dentin interface. Dent Mater 30, e169–e188.CrossRefGoogle ScholarPubMed
Toledano, M., Cabello, I., Yamauti, M., Giannini, M., Aguilera, F.S., Osorio, E. & Osorio, R. (2012 a). Resistance to degradation of resin–dentin bonds produced by one-step self-etch adhesives. Microsc Microanal 18, 1480–1493.CrossRefGoogle ScholarPubMed
Toledano, M., Osorio, E., Aguilera, F.S., Sauro, S., Cabello, I. & Osorio, R. (2014 c). In vitro mechanical stimulation promoted remineralization at the resin/dentin interface. J Mech Behav Biomed Mater 30, 61–74.CrossRefGoogle ScholarPubMed
Toledano, M., Osorio, R., Albaladejo, A., Aguilera, F.S., Tay, F.R. & Ferrari, M. (2006). Effect of cyclic loading on the microtensile bond strengths of total-etch and self-etch adhesives. Oper Dent 31, 25–32.CrossRefGoogle ScholarPubMed
Toledano, M., Sauro, S., Cabello, I., Watson, T. & Osorio, R. (2013). A Zn-doped etch-and-rinse adhesive may improve the mechanical properties and the integrity at the bonded-dentin interface. Dent Mater 29, e142–e152.CrossRefGoogle ScholarPubMed
Toledano, M., Yamauti, M., Ruiz-Requena, M.E. & Osorio, R. (2012 b). A ZnO-doped adhesive reduced collagen degradation favouring dentine remineralization. J Dent 40, 756–765.CrossRefGoogle ScholarPubMed
Wang, C., Wang, Y., Huffman, N.T., Cui, C., Yao, X., Midura, S., Midura, R.J. & Gorski, J.P. (2009). Confocal laser Raman microspectroscopy of biomineralization foci in UMR 106 osteoblastic cultures reveals temporally synchronized protein changes preceding and accompanying mineral crystal deposition. J Biol Chem 284, 7100–7113.CrossRefGoogle ScholarPubMed
Xu, C. & Wang, Y. (2011). Cross-linked demineralized dentin maintains its mechanical stability when challenged by bacterial collagenase. J Biomed Mater Res B Appl Biomater 96, 242–248.CrossRefGoogle ScholarPubMed
Xu, C. & Wang, Y. (2012). Collagen cross linking increases its biodegradation resistance in wet dentin bonding. J Adhes Dent 14, 11–18.Google ScholarPubMed