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Biomarkers Expression in Human Seminiferous Epithelium

Published online by Cambridge University Press:  28 September 2012

R. Sá
Affiliation:
Lab of Cell Biology, UMIB, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, 4099-003
C. Miranda
Affiliation:
Lab of Cell Biology, UMIB, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, 4099-003
C. Leite
Affiliation:
Lab of Cell Biology, UMIB, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, 4099-003
I. Malheiro
Affiliation:
Lab of Cytogenetics, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, 4099-003
F. Carvalho
Affiliation:
Department of Genetics, Faculty of Medicine, University of Porto, Porto, 4200-319
J. Silva
Affiliation:
Centre for Reproductive GeneticsProf. Alberto Barros, Porto, 4100-012
A. Barros
Affiliation:
Department of Genetics, Faculty of Medicine, University of Porto, Porto, 4200-319 Centre for Reproductive GeneticsProf. Alberto Barros, Porto, 4100-012
M. Sousa
Affiliation:
Lab of Cell Biology, UMIB, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, 4099-003

Abstract

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Spermatogenesis is a complex process of cell proliferation, meiosis and differentiation. In order to determine the genetic mechanisms that control this process we need to develop adequate methodologies for the purification of stage-specific germ cells. In this study we aim to evaluate four markers (c-kit, oct-4, Integrin α6 and Integrin β1), used to isolate spermatogonial stem cells in mammals, as possible markers to isolate/sort testicular adult stem and progenitor cells in humans.

Type
Life Sciences
Copyright
Copyright © Microscopy Society of America 2012