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Benchmarking Cryo-EM Single Particle Analysis Workflows at CEMRC

Published online by Cambridge University Press:  22 July 2022

Anil Kumar ,
Kai Cai ,
Matt R. Larson ,
Bryan S. Sibert ,
Keith Thompson ,
Jae E. Yang  and
Elizabeth R. Wright
Anil Kumar
Affiliation:
Cryo-Electron Microscopy Research Centre, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States
Kai Cai
Affiliation:
Cryo-Electron Microscopy Research Centre, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States
Matt R. Larson
Affiliation:
Cryo-Electron Microscopy Research Centre, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States
Bryan S. Sibert
Affiliation:
Cryo-Electron Microscopy Research Centre, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States
Keith Thompson
Affiliation:
Cryo-Electron Microscopy Research Centre, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States
Jae E. Yang
Affiliation:
Cryo-Electron Microscopy Research Centre, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States
Elizabeth R. Wright*
Affiliation:
Cryo-Electron Microscopy Research Centre, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States Morgridge Institute for Research, University of Wisconsin-Madison, Madison, WI, United States
*
*Corresponding author: [email protected]

Abstract

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Type
On Demand - Technical Advances in Cryo-EM
Information
Microscopy and Microanalysis , Volume 28 , Supplement S1 , August 2022 , pp. 1296 - 1298
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Copyright
Copyright © Microscopy Society of America 2022

References

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Laura, K et al. , Frontiers in Molecular Biosciences (2018). doi: 10.3389/fmolb.2018.00050Google Scholar
Naydenova, K et al. , IUCrJ 6 (2019), p.1086. doi.org/10.1107/S2052252519012612CrossRefGoogle Scholar
This work was supported in part by the University of Wisconsin, Madison, the Department of Biochemistry at the University of Wisconsin, Madison, and public health service grant U24 GM139168 to E.R.W. from the NIH. We are grateful for the use of facilities and instrumentation at the Cryo-EM Research Center in the Department of Biochemistry at the University of Wisconsin, Madison.Google Scholar