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Autophagy and Apoptosis in the Midgut Epithelium of Millipedes

Part of: Micrographia Collection

Published online by Cambridge University Press:  20 May 2019

M.M. Rost-Roszkowska ,
J. Vilimová ,
K. Tajovský ,
A. Chachulska-Żymełka ,
A. Sosinka ,
M. Kszuk-Jendrysik ,
A. Ostróżka  and
F. Kaszuba
M.M. Rost-Roszkowska*
Affiliation:
Department of Animal Histology and Embryology, University of Silesia in Katowice, Bankowa 9, 40-007 Katowice, Poland
J. Vilimová
Affiliation:
Department of Zoology, Charles University, Faculty of Science, Viničná 7, 128 44 Prague 2, Czech Republic
K. Tajovský
Affiliation:
Institute of Soil Biology, Biology Centre CAS, Na Sádkách 7, 370 05 České Budějovice, Czech Republic
A. Chachulska-Żymełka
Affiliation:
Department of Animal Histology and Embryology, University of Silesia in Katowice, Bankowa 9, 40-007 Katowice, Poland
A. Sosinka
Affiliation:
Department of Animal Histology and Embryology, University of Silesia in Katowice, Bankowa 9, 40-007 Katowice, Poland
M. Kszuk-Jendrysik
Affiliation:
Department of Animal Histology and Embryology, University of Silesia in Katowice, Bankowa 9, 40-007 Katowice, Poland
A. Ostróżka
Affiliation:
Department of Animal Histology and Embryology, University of Silesia in Katowice, Bankowa 9, 40-007 Katowice, Poland
F. Kaszuba
Affiliation:
Department of Animal Histology and Embryology, University of Silesia in Katowice, Bankowa 9, 40-007 Katowice, Poland
*
*Author for correspondence: M.M. Rost-Roszkowska, E-mail: [email protected]
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Abstract

The process of autophagy has been detected in the midgut epithelium of four millipede species: Julus scandinavius, Polyxenus lagurus, Archispirostreptus gigas, and Telodeinopus aoutii. It has been examined using transmission electron microscopy (TEM), which enabled differentiation of cells in the midgut epithelium, and some histochemical methods (light microscope and fluorescence microscope). While autophagy appeared in the cytoplasm of digestive, secretory, and regenerative cells in J. scandinavius and A. gigas, in the two other species, T. aoutii and P. lagurus, it was only detected in the digestive cells. Both types of macroautophagy, the selective and nonselective processes, are described using TEM. Phagophore formation appeared as the first step of autophagy. After its blind ends fusion, the autophagosomes were formed. The autophagosomes fused with lysosomes and were transformed into autolysosomes. As the final step of autophagy, the residual bodies were detected. Autophagic structures can be removed from the midgut epithelium via, e.g., atypical exocytosis. Additionally, in P. lagurus and J. scandinavius, it was observed as the neutralization of pathogens such as Rickettsia-like microorganisms. Autophagy and apoptosis ca be analyzed using TEM, while specific histochemical methods may confirm it.

Keywords

cell deathdigestive systemDiplopodahistochemistrymidgutultrastructure
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 25 , Issue 4 , August 2019 , pp. 1004 - 1016
Copyright
Copyright© Microscopy Society of America 2019 

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