Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-26T08:43:31.427Z Has data issue: false hasContentIssue false
  • English
  • Français

Attachment and Invasion of Clostridium Difficile Endospores Incubated with CACO-2 Cells

Published online by Cambridge University Press:  02 July 2020

B.J. Panessa-Warren ,
G.T. Tortora  and
J.B. Warren
B.J. Panessa-Warren
Affiliation:
Graduate Division, School of Health Technology & Management, SUNYat , Stony Brook, N.Y.I 1794
G.T. Tortora
Affiliation:
Clinical Microbiology Laboratory, University Hospital SUNY and Dept. of Clinical Lab Sciences, SUNY at , Stony Brook, N.Y.I 1794
J.B. Warren
Affiliation:
Instrumentation Division, Building 535B, Brookhaven National Laboratory, Upton, NY., I 1973
Get access

Extract

The small (0.5-0.75 um) Clostridial endospores are highly resistant to solvents, acids, bases, radiation, heat, cold, pressure, vacuum and most sporicides. C.difficile causes antibiotic induced pseudomembranous colitis, and is responsible for almost 20% of nosocomial diarrheas in todays hospitals. These endospores have been found on hospital floors, sheets and bedding, as well as on the hands and white coats of hospital personnel.

Our previous studies revealed that these endospores attach to agar by using a two phase attachment process. To examine if this mechanism for spore attachment is also responsible for C.difficile pathogenesis in the human colon, spore attachment and invasion of monolayers of human colon carcinoma cells (Caco-2 cells) using two strains of C.difficile,(ATCC 43594 an isolate from a patient with pseudomembranous colitis [FIG.1A] and ATCC 9689 a clinical microbology laboratory quality control strain) was studied. Cell monolayers grown on 6mm coverslips were inoculated with 10 ul of spore suspension (0.5 McFarland turbidity in sterile water),

Type
Microorganisms: The Good, The Bad, The Unusual
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 660 - 661
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

References:

l.Russell, A.,Clin. Microbiol. Rev. 3( 1990)99.CrossRefGoogle Scholar
2.McFarland, L. et al.,New England J. of Med. 320( 1989)204.CrossRefGoogle Scholar
3.Bartlett, J., in Clostridium difficile:lt's Role in Intestinal Disease, Academic Press (1988)1.Google Scholar
4.Lyerly, D. et al., Clin. Microbiol. Rev. l(1988)l.Google Scholar
5.Panessa-Warren, B. et al.,Tissue & Cell,29( 1997)441.CrossRefGoogle Scholar