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Approaches for Understanding Dynamic Cell Movements, Cell-Cell Interactions and Tissue Shaping During Embryogenesis of the Vertebrate Body Plan
Published online by Cambridge University Press: 02 July 2020
Extract
The early vertebrate embryo develops a characteristic tube-within-a-tube body plan. This plan is realized through a series of cell movements and cell-cell interactions that collectively result in tissue shaping and the formation of the three-dimensional body plan. Tissue shaping is a highly choreographed process that is under the control of the organizer--a specialized region of the embryo that is both sufficient and required for formation of the body plan. Recent technical advances have greatly increased our understanding of the role of the organizer in vertebrate embryogenesis. Such advances include the use of new cellular, molecular, genetic, and embryological approaches.
A hallmark of embryogenesis is its dynamic nature. Classically, embryos were studied in three major ways. 1) With morphological/descriptive analysis, initially involving histological procedures (stained whole mounts and serial sections cut in the three cardinal axes) and more recently electron microscopy.
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- Applications of Imaging Techniques to the Study of Embryological Development
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- Copyright © Microscopy Society of America
References
1.Smith, J.L. and Schoenwolf, G.C. 1998 Getting Organized: New Insights into the Organizer of Higher Vertebrates. In: Current Topics in Developmental Biology. Vol. 40. Eds., Pedersen, R.A. and Schatten, G.P.. Academic Press, Inc., Orlando. Pp. 79–110.Google Scholar
2.Darnell, D.K. and Schoenwolf, G.C. 1996 Modern techniques for cell labeling in avian and murine embryos. In: Molecular and Cellular Methods in Developmental Toxicology. Ed, Daston, G.P.. CRC press, Boca Raton, Florida. Pp. 231–273.Google Scholar
3.Darnell, D.K. and Schoenwolf, G.C. 1999 The Chick Embryo as a Model System for Analyzing Mechanisms of Development. In: Methods in Molecular Biology. Developmental Biology Protocols, Vol. I, Chap. 4. Eds., Tuan, R.S. and Lo., C.W.Humana Press, Inc., Totowa, N.J. In press.Google Scholar
4.D.K., Darnell and Schoenwolf, G.C. 1999 Culture of Avian Embryos. In: Methods in Molecular Biology. Developmental Biology Protocols, Vol. I, Chap. 6. Eds., Tuan, R.S. and Lo, C.W.. Humana Press, Inc., Totowa,N.J. In press.Google Scholar
5.D.K., Darnell and Schoenwolf, G.C. 1999 Transplantation Chimeras: Use in Analyzing Mechanisms of Avian Development. In: Methods in Molecular Biology. Developmental Biology Protocols, Vol. I, Chap. 33. Eds., Tuan, R.S. and Lo, C.W.. Humana Press, Inc., Totowa, N.J. In press.Google Scholar
6.Darnell, D.K., Garcia-Martinez, V., Lopez-Sanchez, C., Yuan, S. and Schoenwolf, G.C. 1999 Dynamic Labeling Techniques for Fate Mapping, Testing Cell Commitment and Following Living Cells in Avian Embryos. In: Methods in Molecular Biology. Developmental Biology Protocols, Vol. I, Chap. 37. Eds., Tuan, R.S. and Lo, C.W.. Humana Press, Inc., Totowa, N.J. In press.Google Scholar
7.Schoenwolf, G.C. 1999 The avian embryo: A model for descriptive and experimental embryology. In: Cell Lineage and Fate Determination. Ed., Moody, S.A.. Academic Press, New York.CrossRefGoogle Scholar
8. This research was supported by the National Institutes of Health, Nos. HD 28845 and NS 18112.Google Scholar