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Apoptosis During the Knee Joint Development of Mouse

Published online by Cambridge University Press:  02 July 2020

D. H. Hwang ,
H. S. Lim  and
Y. W. Kim
D. H. Hwang
Affiliation:
Department of Anatomy, Seoul National University College of Medicine, Yongon-Dong 28, Chongno-Gu, Seoul, 110-799Korea
H. S. Lim
Affiliation:
Department of Anatomy, Seoul National University College of Medicine, Yongon-Dong 28, Chongno-Gu, Seoul, 110-799Korea
Y. W. Kim
Affiliation:
Department of Anatomy, Seoul National University College of Medicine, Yongon-Dong 28, Chongno-Gu, Seoul, 110-799Korea
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Extract

Apoptosis has been known as a significant element in the process of embryonic morphogenesis (1). Many studies on the role of apoptosis during development have been done using several organ systems, such as limb bud (2), nervous system(3), and inner ear (4). Recently, cell death with many morphological characteristics of apoptosis was reported in the developmental study of chick knee joint (5). This study was designed to observe the appearance of apoptotic cells during the joint cavity formation of mouse knee joint by transmission electron microscopy and TUNEL staining. The fetus were collected from l5thday(15D) to 19th day(l 9D) of pregnancy.

We observed many blood vessels and mesenchymal cells at 15D between the cartilages of femur and tibia. Mesenchymal cells had condensed heterochromatin and dilated rough endoplasmic reticulum (rER). At 16D, the joint clefts were first formed. Apoptotic cells, with characteristic nuclear fragmentation and condensation, appeared between the joint cleft and the capillaries.

Type
Developmental/Reproductive Biology
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 960 - 961
Copyright
Copyright © Microscopy Society of America

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References

References:

l.Sanders, E.J. and Wride, M. A.. Programmed Cell Death in Development, Int Rev Cytol(1995) 163:105–173Google Scholar
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4.Nishikori, T. et al., Apoptosis During Inner Ear Development in Human and Mouse Embryos: An Analysis by Computer-assisted Three-dimensional Reconstruction, Anal Embryol(1999)200:19–26CrossRefGoogle ScholarPubMed
5.Abu-hijleh, G. et al., Cell Death in the Developing Chick Knee Joint: I. Spatial and Temporal Patterns, Clinic Anat(1997) 10:183–2003.0.CO;2-V>CrossRefGoogle ScholarPubMed