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An Instant Cell Recognition System Using a Microfabricated Coordinate Standard Chip Useful for Combinable Cell Observation with Multiple Microscopic Apparatuses

Published online by Cambridge University Press:  03 March 2008

Yohei Yamada ,
Naotoshi Yamaguchi ,
Masakazu Ozaki ,
Yukihiro Shinozaki ,
Mikako Saito  and
Hideaki Matsuoka
Yohei Yamada
Affiliation:
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan Chuo Precision Industrial Co. Ltd., Oikawa Building, Kanda Awaji-cho 1-5, Chiyoda-ku, Tokyo 101-0063, Japan CREST (Core Research for Evolutional Science and Technology), Japan Science and Technology Agency, Honcho 4-1-8, Kawaguchi, Saitama 332-0012, Japan
Naotoshi Yamaguchi
Affiliation:
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan CREST (Core Research for Evolutional Science and Technology), Japan Science and Technology Agency, Honcho 4-1-8, Kawaguchi, Saitama 332-0012, Japan
Masakazu Ozaki
Affiliation:
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
Yukihiro Shinozaki
Affiliation:
Chuo Precision Industrial Co. Ltd., Oikawa Building, Kanda Awaji-cho 1-5, Chiyoda-ku, Tokyo 101-0063, Japan CREST (Core Research for Evolutional Science and Technology), Japan Science and Technology Agency, Honcho 4-1-8, Kawaguchi, Saitama 332-0012, Japan
Mikako Saito
Affiliation:
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan CREST (Core Research for Evolutional Science and Technology), Japan Science and Technology Agency, Honcho 4-1-8, Kawaguchi, Saitama 332-0012, Japan
Hideaki Matsuoka*
Affiliation:
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan CREST (Core Research for Evolutional Science and Technology), Japan Science and Technology Agency, Honcho 4-1-8, Kawaguchi, Saitama 332-0012, Japan
*
Corresponding author. E-mail: [email protected]
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Abstract

Disposable coordinate standard (CS) chips were fabricated by the ejection of melted polystyrene into a metal mold. The CS chip surface was divided into four parts different in height and width. The edge lines of these parts could be recognized as straight lines 2 μm in width in the microscope view and used as the X and Y axes for the culture dish. The CS chip was attached on the bottom of a culture dish outside. Then the dish was set on the microscope stage and moved by means of a motorized automatic stage. The X-Y coordinates of many single-cells in a culture dish were registered, respectively. Once registered, any single-cell could instantly be brought to the center of the microscope view even after displacing the dish from the stage for a while and setting it again on the stage. Therefore, experimenters can easily search any single-cell in any culture dish on any microscope at any time. Such a system is remarkably useful for various modes of single-cell experiment and named “Suguwaculture,” which means “instantly” (“sugu” in Japanese) + “recognizable” (“wakaru” in Japanese) + “culture” (during culture).

Keywords

instant cell recognitioncoordinate standard chipX-Y coordinates registrationcombinable observation on multiple microscopesSuguwaculturesingle-cell experimenthigh throughput microinjectionmicroscopic image analysis
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 14 , Issue 3 , June 2008 , pp. 236 - 242
Copyright
Copyright © Microscopy Society of America 2008

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