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An Electron Microscopic Study of Developing Synapses in Facial and Hypoglossal Motor Nuclei of the Brazilian Opossum.

Published online by Cambridge University Press:  02 July 2020

T.M. Pepper ,
J.J. Swanson ,
M.C. Kuehl-Kovarik  and
CD. Jacobson
T.M. Pepper
Affiliation:
Department of Veterinary Anatomy, Iowa State University, Ames, IA, 50011.
J.J. Swanson
Affiliation:
Department of Veterinary Anatomy, Iowa State University, Ames, IA, 50011.
M.C. Kuehl-Kovarik
Affiliation:
Department of Veterinary Anatomy, Iowa State University, Ames, IA, 50011.
CD. Jacobson
Affiliation:
Department of Veterinary Anatomy, Iowa State University, Ames, IA, 50011.
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Extract

Previously, we have shown the apparent development of synapses utilizing immunohistochemistry (IHC) for synapse-associated proteins in the facial and hypoglossal motor nuclei of the Brazilian opossum. This study suggests that synaptogenesis is delayed in the facial motor nucleus (FMN) as compared to the hypoglossal motor nucleus (HMN). In the present study we plan to confirm and extend these findings at the electron microscopic (EM) level.

We have examined the ultrastructure of the developing FMN from animals 5 to 25 days of postnatal age (PN). The specific nuclei were identified using methylene blue stained 150 μm thick vibratome cut sections. The FMN and HMN were then dissected out for processing, guaranteeing that visualization at the EM level would be confined to areas specific to the nuclei of interest.

Initial analysis of the FMN indicates a marked increase of detectable synapses between 5 and 15PN.

Type
Developmental and Reproductive Biology
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 181 - 182
Copyright
Copyright © Microscopy Society of America 1997

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References

1.Swanson, J.J.et al., Journal of Comparative Neurology, 368(1996).Google Scholar
2.Dellmann, H.D.et al., Stain Technology 58:6 (1983).10.3109/10520298309066806CrossRefGoogle Scholar
3. The authors wish to thank Jean, Olson with the Veterinary Medicine Research Institute at Iowa State University and Dr.T. Horner, Harry with the Bessey Microscopy Facility at Iowa State University. This work was funded by The Whitehall Foundation and NSF.Google Scholar