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Wide-Field Deconvolution for Time-Lapse 3D Microscopy Of Cell Locomotion: The Good, The Bad and the Artifactual

Published online by Cambridge University Press:  02 July 2020

J.G. McNally
J.G. McNally*
Affiliation:
Institute for Biomedical Computing, Washington University, St. Louis, MO., 63110
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Extract

We are using wide-field deconvolution microscopy to study the locomotion of cells within a three-dimensional cell mass. How cells move within such an environment is poorly understood, even though such movement occurs widely, for example, during embryonic development or tumor cell invasion. In these and other cases, cells moving within a cell mass face challenges not encountered by isolated cells crawling on a flat substrate. Cells within a mass must clear a path for themselves, and also gain traction on other cells which themselves may be moving in the same or another direction. To understand the mechanisms of such 3D movement, we observe and analyze it in both normal and mutant cells. The mutant analysis sheds light on which molecules are important for 3D locomotion.

To visualize either normal or mutant cell locomotion within a three-dimensional cell mass, we require time-lapse 3D imaging at light levels low enough to maintain specimen viability.

Type
Deconvolution of Biological Images for 3D Light Microscopy—Confocal & Widefield
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 884 - 885
Copyright
Copyright © Microscopy Society of America

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References

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