The annual Microscopy & Microanalysis meeting has proven to be the premiere meeting for scientists, technologists, and students who use microscopy or microanalysis in their research. Microscopy & Microanalysis 2009 will be no exception! The 2009 Executive Program Committee, consisting of co-chairs from each of the participating societies, has assembled a diverse and comprehensive program that will address applications in both the biological and physical sciences as well as recent and emerging trends in instrumentation and techniques. As in 2008, special emphasis has been placed on attracting fresh content and new faces. Several symposia have been designed to expand beyond the boundaries of the interdisciplinary nature of the Microscopy & Microanalysis meetings, reflecting the increasing need for collaborations between physical and biological sciences.