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The Ultrastructure of Cadaverous Skin after Cryopreservation with Polyethylene Oxide-400

Published online by Cambridge University Press:  02 July 2020

R. Migunova ,
B. Sandomirski  and
A. Kaprelyants
R. Migunova
Affiliation:
Department of cryocytology, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, 61015, Ukraine Email:[email protected]
B. Sandomirski
Affiliation:
Department of cryomedicine, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, 61015, Ukraine
A. Kaprelyants
Affiliation:
Department of cryocytology, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, 61015, Ukraine Email:[email protected]
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Extract

The cryopreservation is the method of selection of long-term skin preservation used for the closure of large wound defects and the only possibility of long preservation of graft skin material in the viable state. For the lowtemperature preservation of human skin was successfully used cryoprotectant Polyethylene oxide -400, which was confirmed by the clinical evaluation during autotransplantation /1/. At the same time many aspects of the influence of low temperatures on the ultrastructural skin components were unclear /2/. The investigation of subcellular elements during step of low-temperature preservation specifies mechanisms of influence on the tissue preservation factors and determine the condition of skin cellular elements /3,4,5/. The aim of this work is to study ultrastructural characteristics of human cadaverous skin during the stages of low-temperature preservation by PEO-400.

In this investigation epidermal skin grafts with the thickness of 0.4-0.6 mm were studied in 6 hours after the accidental death divided in 3 series:

Type
Pathology
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 592 - 593
Copyright
Copyright © Microscopy Society of America

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References

References:

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