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Three-Dimensional Morphological and Mineralogical Characterization of Testate Amebae

Published online by Cambridge University Press:  10 September 2013

Eric Armynot du Châtelet ,
Catherine Noiriel  and
Maxence Delaine
Eric Armynot du Châtelet*
Affiliation:
Université de Lille I, CNRS, Laboratoire Géosystèmes, 59655 Villeneuve d'Ascq, France
Catherine Noiriel
Affiliation:
Géosciences Environnement Toulouse (GET), Observatoire Midi-Pyrénées, Université Paul Sabatier, CNRS, IRD, 31400 Toulouse, France
Maxence Delaine
Affiliation:
Université de Lille I, CNRS, Laboratoire Géosystèmes, 59655 Villeneuve d'Ascq, France
*
*Corresponding author. E-mail: [email protected]
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Abstract

Testate amebae are unicellular shelled protozoa commonly used as indicators in ecological and paleoecological studies. We explored the potential application of three-dimensional (3D) X-ray micro-tomography used in addition to 2D techniques (environmental scanning electron microscopy, electron probe micro-analysis, and cathodoluminescence) for detailed characterization of agglutinated shells of protozoa. We analyzed four specimens of the aquatic testate ameba Difflugia oblonga (Arcellinida), to test whether size distribution and mineral composition of shell grains diverged from sediment size distribution and mineralogical composition. From the 3D images, the geometry of the specimens (size and mass) and of the individual grains forming the specimen (grain size distribution and volume) were calculated. Based on combined chemical, mineralogical, and morphological analyses we show that D. oblonga is able to selectively pick up the small size fraction of the sediment with a preference for low-density silicates close to quartz density (~2.65). The maximum size of the grains matches the size of the pseudostome (shell aperture), suggesting the existence of a physical limit to grain size used for building the shell. This study illustrates the potential of this combined approach to characterize agglutinated shells of protozoa. This data can be useful for detailed morphological studies with applications in taxonomy and ecology.

Keywords

testate amebae3D morphologyX-ray micro-tomographycathodoluminescenceelectron microprobe analysisenvironmental scanning electron microscope
Type
Biomedical and Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 6 , December 2013 , pp. 1511 - 1522
Copyright
Copyright © Microscopy Society of America 2013 

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