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Three Dimensional Optical Data Storage Using Two-Photon Induced Photobleaching in a Polymer Matrix

Published online by Cambridge University Press:  02 July 2020

S. J. Pan ,
A. Shih ,
M. S. Park ,
W. S. Liou ,
J. Bhawalkar ,
P. Prasard  and
P. C. Cheng
S. J. Pan
Affiliation:
Advanced Microscopy and Imaging Laboratory, Dept. of Electrical and Computer Eng., State Univ. of New York, Buffalo, NY, 14260USA
A. Shih
Affiliation:
Advanced Microscopy and Imaging Laboratory, Dept. of Electrical and Computer Eng., State Univ. of New York, Buffalo, NY, 14260USA
M. S. Park
Affiliation:
Advanced Microscopy and Imaging Laboratory, Dept. of Electrical and Computer Eng., State Univ. of New York, Buffalo, NY, 14260USA
W. S. Liou
Affiliation:
Advanced Microscopy and Imaging Laboratory, Dept. of Electrical and Computer Eng., State Univ. of New York, Buffalo, NY, 14260USA
J. Bhawalkar
Affiliation:
Dept. of Chemistry, State Univ. of New York, Buffalo, NY, 14260, USA
P. Prasard
Affiliation:
Dept. of Chemistry, State Univ. of New York, Buffalo, NY, 14260, USA
P. C. Cheng
Affiliation:
Advanced Microscopy and Imaging Laboratory, Dept. of Electrical and Computer Eng., State Univ. of New York, Buffalo, NY, 14260USA
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Extract

The intensity of two-photon induced fluorescent shows quadratic dependency to the excitation intensity, as a result, one can achieve optical sectioning in fluorescent microscopy without the use of a confocal aperture. When the excitation power is properly adjusted, the fluorescent and photobleached volume is restricted to the vicinity of the focal points; therefore, it is possible to use this mechanism to achieve 3D data storage and microstructure fabrication (stereolithography).

The recording medium used in this experiment was poly-HEMA block doped with a high efficient two-photon fluorophore, APSS, (4-[N-(2-hydroxyethyl)-N-methyl) amino phenyl]-4’-(6-hydroxyhexyl sulfonyl) stilbene). The block was made by dissolving APSS in HEMA with an initiator, 2,2’-azobisisobutyronitril (AiBN) (1% mole ratio) and polymerized at 60° C for 48 hours. The polymer was then trimmed into a small rectangular block (3×5×1 mm3) and surfaced by using a ultramicrotome with a glass knife. A modified Biorad MC500 LSM equipped with a home-made computer controlled stage scanner was used for both writing (stage scanning) and read-back (beam scanning).

Type
Biological Applications of Multi-photon Excitation Fluorescence Imaging
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 303 - 304
Copyright
Copyright © Microscopy Society of America 1997

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References

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