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Telocytes in Different Organs of Vertebrates: Potential Essence Cells of the Meridian in Chinese Traditional Medicine

Part of: Micrographia Collection

Published online by Cambridge University Press:  11 May 2020

Shi Yonghong ,
Wu Ruizhi ,
Zhang Yue ,
Bai Xuebing ,
Imran Tarique ,
Liang Chunhua ,
Yang Ping  and
Chen Qiusheng Open the ORCID record for Chen Qiusheng [Opens in a new window]
Shi Yonghong
Affiliation:
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province210095, China
Wu Ruizhi
Affiliation:
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province210095, China
Zhang Yue
Affiliation:
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province210095, China
Bai Xuebing
Affiliation:
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province210095, China
Imran Tarique
Affiliation:
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province210095, China
Liang Chunhua
Affiliation:
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province210095, China
Yang Ping
Affiliation:
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province210095, China
Chen Qiusheng*
Affiliation:
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province210095, China
*
*Author for correspondence: Chen Qiusheng, E-mail: [email protected]
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Abstract

Telocytes (TCs) are very long, non-neuronal, somatic cells whose function is widely believed to be involved in providing connections between different cells within the body. The cellular characteristics of TCs in various organs have been studied by immunohistochemistry, double immunofluorescence and electron microscopy in different vertebrate species, and here we investigate the proposed properties of these cells in the context of the “meridian” in Chinese Traditional Medicine (CTM). The results show that TCs and their long extensions, telopodes (Tps) develop a complicated network by homo- and heterocellular junctions in the connective tissue throughout the body, which can connect the skin with distant organs. In concept, this is the analogue of ancient meridian maps connecting skin acupoints with the viscera. Various active cells and extracellular vesicles including exosomes move along Tps, which, along with developed mitochondria within the podoms of Tps, may account for the structural evidence for “Qi” (vital energy and signal communication) in CTM. Morphological associations of TCs with the nerve, vascular, endocrine, and immune systems are also compatible with previously proposed meridian theories in CTM. Close relationships exist between TCs and collagen fiber bundles and some structures in skin fascia provide the microanatomical support for acupuncture treatment based on the meridian principle. The dynamicity in the distribution and structure of TCs reflects the plasticity of the meridian at the cellular level. As the same attribute, both the meridian and the TC have been associated with various diseases. Here, we summarize structural analogues between the TC and the meridian, suggesting that TCs have the cytological characteristics of the CTM meridian. We, therefore, hypothesize that TCs are the “essence cells” of the CTM meridian, which can connect and integrate different cells and structures in the connective tissue.

Keywords

Chinese traditional medicineessence cellmeridianmorphologytelocyte
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 26 , Issue 3 , June 2020 , pp. 575 - 588
Copyright
Copyright © Microscopy Society of America 2020

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References

Ahn, AC, Colbert, AP, Anderson, BJ, Martinsen, ØG, Hammerschlag, R, Cina, S, Wayne, PM & Langevin, HM (2008). Electrical properties of acupuncture points and meridians: A systematic review. Bioelectromagnetics 29(4), 245–256.CrossRefGoogle ScholarPubMed
Ahn, AC, Park, M, Shaw, JR, McManus, CA, Kaptchuk, TJ & Langevin, HM (2010). Electrical impedance of acupuncture meridians: The relevance of subcutaneous collagenous bands. PLoS ONE 5, 7.CrossRefGoogle ScholarPubMed
Aleksandrovych, V, Pasternak, A, Basta, P, Sajewicz, M, Walocha, J & Gil, K (2017). Telocytes: Facts, speculations and myths (Review article). Folia Med Cracov 57, 5–22.Google Scholar
Ceafalan, L, Gherghiceanu, M, Popescu, LM & Simionescu, O (2012). Telocytes in human skin – Are they involved in skin regeneration? J Cell Mol Med 16(7), 1405–1420.CrossRefGoogle ScholarPubMed
Chaitow, L (2017). Telocytes: connective tissue repair and communication cells. J Bodyw Mov Ther 21, 231–233.CrossRefGoogle ScholarPubMed
Cismaşiu, VB & Popescu, LM (2015). Telocytes transfer extracellular vesicles loaded with microRNAs to stem cells. J Cell Mol Med 19(2), 351–358.CrossRefGoogle ScholarPubMed
Cretoiu, D, Gherghiceanu, M, Hummel, E, Zimmermann, H, Simionescu, O & Popescu, LM (2015). FIB-SEM tomography of human skin telocytes and their extracellular vesicles. J Cell Mol Med 19(4), 714–722.CrossRefGoogle ScholarPubMed
Dawidowicz, J, Szotek, S, Matysiak, N, Mielańczyk, Ł & Maksymowicz, K (2015). Electron microscopy of human fascia lata: Focus on telocytes. J Cell Mol Med 19(10), 2500–2506.CrossRefGoogle ScholarPubMed
Felisbino, SL, Sanches, BDA, Delella, FK, Scarano, WR, Dos Santos, FCA, Vilamaior, PSL, Taboga, SR & Justulin, LA (2019). Prostate telocytes change their phenotype in response to castration or testosterone replacement. Sci Rep 9(1), 3761.CrossRefGoogle ScholarPubMed
Ghiron, C (2019). The primo vascular system as a possible exosomal route across the body: implications for tumor proliferation and metastasis. J Acupunct Meridian Stud 12(1), 25–28.CrossRefGoogle ScholarPubMed
Hua, P, Lü, H, Yuan, L & Tang, L (2006). Four main schools of thought and analysis in studies of channels and collaterals. Zhongguo Zhen Jiu 26(6), 407–413.Google ScholarPubMed
Janas, P, Kucybała, I, Radoń-Pokracka, M & Huras, H (2018). Telocytes in the female reproductive system: An overview of up-to-date knowledge. Adv Clin Exp Med 27(4), 559–565.CrossRefGoogle ScholarPubMed
Kimura, M, Tohya, K, Kuroiwa, K-i, Oda, H, Gorawski, EC, Zhong, XH, Toda, S, Ohnishi, M & Noguchi, E (1992). Electron microscopical and immunohistochemical studies on the induction of “Qi” employing needling manipulation. Am J Chin Med 20(01), 25–35.CrossRefGoogle ScholarPubMed
Kovich, F (2019). A new definition of an acupuncture meridian. J Acupunct Meridian Stud 12(1), 37–41.CrossRefGoogle ScholarPubMed
Langevin, HM & Yandow, JA (2002). Relationship of acupuncture points and meridians to connective tissue planes. Anat Rec 269(6), 257–265.CrossRefGoogle ScholarPubMed
Lee, Y-C, Ng, HP, Chang, Y-H & Ho, W-C (2018). The development and application evaluation of meridian energy detection system in traditional oriental medicine: A preliminary study. J Evid Based Complementary Altern Med 2018, 9469703.Google ScholarPubMed
Li, N-c, Li, M-y, Chen, B & Guo, Y (2019). A new perspective of acupuncture: The interaction among three networks leads to neutralization. J Evid Based Complementary Altern Med 2019, 2326867.Google ScholarPubMed
Maurer, N, Nissel, H, Egerbacher, M, Gornik, E, Schuller, P & Traxler, H (2019). Anatomical evidence of acupuncture meridians in the human extracellular matrix: Results from a macroscopic and microscopic interdisciplinary multicentre study on human corpses. J Evid Based Complementary Altern Med 2019, 6976892.Google ScholarPubMed
Mingfu, L, Xiaotong, D, Xiaojing, S, Jin, J, Jinling, Z & Ying, H (2013). Study on the dynamic compound structure composed of mast cells, blood vessels, and nerves in rat acupoint. J Evid Based Complementary Altern Med 2013, 160651.Google Scholar
Nawaz, M, Shah, N, Zanetti, BR, Maugeri, M, Silvestre, RN, Fatima, F, Neder, L & Valadi, H (2018). Extracellular vesicles and matrix remodeling enzymes: The emerging roles in extracellular matrix remodeling, progression of diseases and tissue repair. Cells 7(10), 167.CrossRefGoogle ScholarPubMed
Popescu, LM & Faussone-Pellegrini, M-S (2010). TELOCYTES – A case of serendipity: The winding way from Interstitial Cells of Cajal (ICC), via Interstitial Cajal-Like Cells (ICLC) to TELOCYTES. J Cell Mol Med 14(4), 729–740.CrossRefGoogle Scholar
Rasera Zotelli, VL, Grillo, CM, Bressiani Gil, ML, Wada, RS, Sato, JE & de Sousa, MdLR (2018). Patterns of energy imbalance of the meridians in patients with temporomandibular dysfunction. J Acupunct Meridian Stud 11(1), 1–6.CrossRefGoogle ScholarPubMed
Samuelson, LC (2018). Debate over the identity of an intestinal niche-cell population settled. Nature 558(7710), 380–381.CrossRefGoogle ScholarPubMed
Shim, W (2016). Myocardial telocytes: a new Player in electric circuitry of the heart. Adv Exp Med Biol 913, 241–251.CrossRefGoogle ScholarPubMed
Shoshkes-Carmel, M, Wang, YJ, Wangensteen, KJ, Tóth, B, Kondo, A, Massasa, EE, Itzkovitz, S & Kaestner, KH (2018). Subepithelial telocytes are an important source of Wnts that supports intestinal crypts. Nature 557(7704), 242–246.CrossRefGoogle ScholarPubMed
Stefanov, M, Potroz, M, Kim, J, Lim, J, Cha, R & Nam, M-H (2013). The primo vascular system as a new anatomical system. J Acupunct Meridian Stud 6(6), 331–338.10.1016/j.jams.2013.10.001CrossRefGoogle ScholarPubMed
Stzepourginski, I, Nigro, G, Jacob, J-M, Dulauroy, S, Sansonetti, PJ, Eberl, G & Peduto, L (2017). CD34+ mesenchymal cells are a major component of the intestinal stem cells niche at homeostasis and after injury. Proc Natl Acad Sci USA 114(4), E506–E513.CrossRefGoogle ScholarPubMed
Varga, I, Polák, Š, Kyselovič, J, Kachlík, D, Danišovič, Ľ & Klein, M (2019). Recently discovered interstitial cell population of telocytes: Distinguishing facts from fiction regarding their role in the pathogenesis of diverse diseases called “telocytopathies”. Medicina (Kaunas) 55(2), 56.CrossRefGoogle ScholarPubMed
Xinnong, C (1987). Chinese Acupuncture and Moxibustion. Beijing: Foreign Languages Press.Google Scholar
Yang, W (1994). Huangdi nei jing ling shu shi jie. Zhi Yuan Shu Ju (Taiwan), pp. 25–32.Google Scholar
Zhang, B, Yang, C, Qiao, L, Li, Q, Wang, C, Yan, X and Lin, J (2017). Telocytes: a potential defender in the spleen of Npc1 mutant mice. J Cell Mol Med 21, 848–859.CrossRefGoogle ScholarPubMed
Zhou, W & Benharash, P (2014). Effects and mechanisms of acupuncture based on the principle of meridians. J Acupunct Meridian Stud 7(4), 190–193.CrossRefGoogle ScholarPubMed
Zhu, B (2015). The plasticity of acupoint. Zhongguo Zhen Jiu 35(11), 1203–1208.Google ScholarPubMed