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Subcellular Distribution Of Basic Fibroblast Growth Factor-Like Molecules In Fibroblasts And Extracellular Matrix

Published online by Cambridge University Press:  02 July 2020

R. G. Aktas  and
R. J. Kayton
R. G. Aktas
Affiliation:
Trakya University, Faculty of Medicine, Pathology Department, Edirne, TURKEY
R. J. Kayton
Affiliation:
Oregon Health Sciences University, C.R.O.E.T., Portland, OR, U.S.A.
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Extract

Basic fibroblast growth factor (bFGF) is thought to play an important role in normal tissue repair and wound healing. It is a potent mitogenic and chemotactic factor for fibroblasts. To some degree bFGF regulates proliferation and extracellular matrix (ECM) production by these cells. In this study we investigate (i) the distribution of bFGF in fibroblasts and ECM of supporting tissues (ii) the subcellular location of this protein in vivo and, (iii) mechanisms for release of this growth factor from fibroblasts.

Spurr's resin embedded ultrathin sections from adult rat sciatic nerve, adrenal gland, kidney; and from normal human lung and skin were labeled with either polyclonal (F3393-Sigma) or monoclonal antibodies (F6162-Sigma, C3316-ZymoGenetics Inc.) specific for bFGF using a two-step immunogold labeling method. Controls were incubated with either an equivalent volume of blocking solution (omitting the primary antibody) or an irrelevant antibody (Factor VIII, anti-Histamine)(Fig. 4).

Type
Cytochemistry
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1332 - 1333
Copyright
Copyright © Microscopy Society of America

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References

References:

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