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Short-Term Response of Mitomycin C on the Human Rectus Muscle Following Strabismus Surgery: Histological, Ultrastructural, and Biomechanical Evaluation

Published online by Cambridge University Press:  04 February 2013

Samjin Choi ,
Youjin Cheong ,
Jae-Ho Shin ,
Kyung A Kim ,
Jae Beum Bang ,
Kyung-Hyun Jin  and
Hun-Kuk Park
Samjin Choi
Affiliation:
Department of Biomedical Engineering & Healthcare Industry Research Institute, Kyung Hee University, Seoul, Korea Department of Orthodontics, Kyung Hee University, Seoul, Korea
Youjin Cheong
Affiliation:
Department of Biomedical Engineering & Healthcare Industry Research Institute, Kyung Hee University, Seoul, Korea
Jae-Ho Shin
Affiliation:
Department of Ophthalmology, Kyung Hee University, Seoul, Korea
Kyung A Kim
Affiliation:
Department of Orthodontics, Kyung Hee University, Seoul, Korea
Jae Beum Bang
Affiliation:
Department of Dental Education, Kyung Hee University, Seoul, Korea
Kyung-Hyun Jin
Affiliation:
Department of Ophthalmology, Kyung Hee University, Seoul, Korea
Hun-Kuk Park*
Affiliation:
Department of Biomedical Engineering & Healthcare Industry Research Institute, Kyung Hee University, Seoul, Korea Department of Medical Engineering, Kyung Hee University, Seoul, Korea
*
*Corresponding author. E-mail: [email protected]
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Abstract

This study investigated the inflammatory effect of intraoperative mitomycin C (MMC) on adhesion reformation in human rectus muscles. Ten consecutive patients who underwent medial rectus resection had their postoperative rectus muscles divided into two groups: control group (n = 10) and MMC group (n = 10). In the MMC group, the muscle was soaked for 2 min with MMC, prepared as a 0.2 mg/mL (0.02%) solution. The 0.02% MMC reactions were examined using histological analysis with hematoxylin-eosin (inflammatory response) and Masson's trichrome (collagen fibrils), immunoreactivities of cyclooxygenase-II (inflammatory response), and collagen type I and III, scanning electron microscopy analysis to quantify the diameter and D-periodicity of collagen fibrils, and atomic force microscopy analysis to quantify the diameter, D-periodicity, and adhesion force of collagen fibrils. The rectus muscles treated with 0.02% MMC showed a significantly increased inflammatory response (p < 0.05), increased collagen density (p < 0.0001), increased fibril diameter (p < 0.001 or p < 0.05), and decreased fibril adhesion force (p < 0.005) compared to the rectus muscles in the control group. MMC simultaneously caused an inflammatory response as well as nanostructural and biomechanical property changes in the collagen fibril network.

Keywords

mitomycin C (MMC)human rectus musclescollagen fibrilsnanostructural and biomechanical propertiesinflammation
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 1 , February 2013 , pp. 227 - 232
Copyright
Copyright © Microscopy Society of America 2013

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