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Scanning Electron and Confocal Scanning Laser Microscopy Imaging of the Ultrastructure and Viability of Vaginal Candida albicans and Non-Albicans Species Adhered to an Intrauterine Contraceptive Device

Published online by Cambridge University Press:  31 August 2010

Luciene C. Farias Paiva ,
Lucélia Donatti ,
Eliana V. Patussi ,
Terezinha I.E. Svizdinski  and
Márcia E. Lopes-Consolaro
Luciene C. Farias Paiva
Affiliation:
Department of Clinical Analysis, Universidade Estadual de Maringá, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil
Lucélia Donatti
Affiliation:
Department of Cell Biology, Universidade Federal do Paraná, Curitiba, 81531-970, Paraná, Brazil
Eliana V. Patussi
Affiliation:
Department of Clinical Analysis, Universidade Estadual de Maringá, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil
Terezinha I.E. Svizdinski
Affiliation:
Department of Clinical Analysis, Universidade Estadual de Maringá, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil
Márcia E. Lopes-Consolaro*
Affiliation:
Department of Clinical Analysis, Universidade Estadual de Maringá, Av. Colombo, 5790, 87020-900, Maringá, Paraná, Brazil
*
Corresponding author. E-mail: [email protected]
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Abstract

Although bacterial biofilms have been studied in detail, adhesion of Candida albicans and non-albicans species to an intrauterine contraceptive device (IUD) is not clear. The objective of this study was to evaluate aspects of imaging of the ultrastructure and viability of vaginal yeasts adhered to different parts of an IUD, through scanning electron microscopy (SEM) and confocal scanning laser microscopy (CSLM). We studied yeasts isolated from different patients with vulvovaginal candidiasis: C. albicans, C. glabrata, C. guillermondii, C. parapsilosis, C. tropicalis, and Saccharomyces cerevisiae. A suspension of the each yeast was prepared and incubated with IUD parts (tail, without copper, and copper-covered). SEM and CSLM showed that all the vaginal yeasts adhered to all the parts of the IUD and demonstrated viability, including 30 days after contact for C. albicans. Possibly irregularities of IUD surface contribute to the adherence process. Although all of the IUD parts contribute to retention of yeasts in the genital tract, high concentration of yeast cells on the tail may indicate the importance of this segment in maintaining the colonization by yeast cells because the tail forms a bridge between the external environment, the vagina that is colonized by yeast cells, and the upper genital tract where there is no colonization.

Keywords

vaginal yeastsintrauterine contraceptive deviceimageadhesionultrastructureviability
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 16 , Issue 5 , October 2010 , pp. 537 - 549
Copyright
Copyright © Microscopy Society of America 2010

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