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The Role of Superoxide and Nitric Oxide in the Development of Myocardial Injury in Rat Myocarditis.

Published online by Cambridge University Press:  02 July 2020

T. Nishikawa ,
S. Ishiyama ,
A. Suzuki ,
A. Masuda  and
T. Kasajima
T. Nishikawa
Affiliation:
Department of Pathology, Tokyo Women's Medical University, Tokyo, 162-8666, Japan
S. Ishiyama
Affiliation:
Department of Pathology, Tokyo Women's Medical University, Tokyo, 162-8666, Japan
A. Suzuki
Affiliation:
Department of Pathology, Tokyo Women's Medical University, Tokyo, 162-8666, Japan
A. Masuda
Affiliation:
Department of Pathology, Tokyo Women's Medical University, Tokyo, 162-8666, Japan
T. Kasajima
Affiliation:
Department of Pathology, Tokyo Women's Medical University, Tokyo, 162-8666, Japan
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Extract

The mechanism of myocardial injury in myocarditis is still unclear. Recently, nitric oxide (NO), massively produced in the inflammation, has been suggested to be harmful for tissue at excess amount as well as superoxide. However, it is still unclear the relationship between production of NO with superoxide and myocardial damage in myocarditis. In this study, we investigated whether NO and/or superoxide play an important role in the pathogenesis of myocardial injury in myocarditis.

The heart tissue specimens were taken from Lewis rats with experimentally induced myocarditis. Immunohistochemical staining was performed using polyclonal antibody for inducible NO synthase by avidin-biotin peroxidase complex method in paraffin sections of the tissue. Histochemical study for ultrastructural H2O2-producing site in the heart tissue was carried out by Brigg’ s method using CeCl3; briefly, the heart tissues were incubated in 0.1M Tris-malate buffer with ImM CeCl3 and 10mM aminotriazole. Then tissues were fixed in 2% glutaraldehyde, postfixed with 1% OsO4 and embedded in resin for electron microscopy.

Type
Biological Specimen Preparation/Cytochemistry/ Immunolabeling/Immunocytochemistry
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 488 - 489
Copyright
Copyright © Microscopy Society of America

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References

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6. This study was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan (No. 11670714), by a grant from the Japan Private School Promotion Foundation and by a grant from the Japan Research Promotion Society for Cardiovascular Disease.Google Scholar