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Role of Reactive Oxygen Species and NFKB p65 in the Lupus Kidney

Published online by Cambridge University Press:  02 July 2020

M. Nishikawa ,
R. Igarashi ,
T. Nakazawa  and
E. Aikawa
M. Nishikawa
Affiliation:
Department of Anatomy and Developmental Biology, Tokyo Women's Medical University, Tokyo, 162-8666
R. Igarashi
Affiliation:
Institute of Medical Science, St Marianna University, Miyamae-ku, Kawasaki, 216-8511, Japan
T. Nakazawa
Affiliation:
Department of Anatomy and Developmental Biology, Tokyo Women's Medical University, Tokyo, 162-8666
E. Aikawa
Affiliation:
Department of Anatomy and Developmental Biology, Tokyo Women's Medical University, Tokyo, 162-8666
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Extract

Reactive oxygen species (ROS) play an important role in lupus nephritis. It has been known that nuclear factor kB(NFkB) is activated by ROS, and binds to KB element in nucleus. Afterwards, inflammatory cytokines are translated and produced in lymphocytes, macrophages and glomerular cells in the kidney. In this study, we investigated the role of ROS and NFkB p65 in the pathogenesis of lupus nephritis (MRL lpr/lpr mice) by the histochemical and immuno-electron microscopic methods.

A modified Brigg’s method was used to determine the generation of H2O2 in the renal tissue of lupus mice. Tissue samples were incubate in 0.1 MTris maleate buffer (pH 7.4) with 7% sucrose, 1mM CeCl3 and 10 mM aminotriazole at 37 °C for 30 min. After completion of the cytochemical reaction, tissues were fixed in 2% w/v glutaraldehyde in 0.1 M cacodylate buffer (pH 7.4) at 4 °C for 60 min.

Type
Biological Specimen Preparation/Cytochemistry/ Immunolabeling/Immunocytochemistry
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 486 - 487
Copyright
Copyright © Microscopy Society of America

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References

References:

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