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Role of Calcium Currents in E-C Coupling and Evolution of the T-System and SR in Developing Cultured Embryonic Amphibian Skeletal Muscle Cells

Published online by Cambridge University Press:  02 July 2020

Gonzalez-Serratos H ,
Cordoba-Rodriguez R ,
Matteson D.R.  and
Rozycka M.
Gonzalez-Serratos H
Affiliation:
Department of Physiology, School of Medicine, University of Maryland, Baltimore, MD21201USA
Cordoba-Rodriguez R
Affiliation:
Department of Physiology, School of Medicine, University of Maryland, Baltimore, MD21201USA
Matteson D.R.
Affiliation:
Department of Physiology, School of Medicine, University of Maryland, Baltimore, MD21201USA
Rozycka M.
Affiliation:
Department of Physiology, School of Medicine, University of Maryland, Baltimore, MD21201USA
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Extract

Adult frog phasic skeletal muscle cells have slow inward calcium current (ICa) (Stanfield, 1977) carried through L-type voltage dependent calcium channels. It has been suggested that ICa may play a role in E-C coupling (Cota & Stefani, 1981, 1989). However, phasic skeletal muscle cells contract for several minutes after the extracellular Ca2+ concentration ([Ca2+]o) is lowered to <10-8 M (Armstrong et al, 1972). Therefore, extracellular Ca2+ (Ca2+o) is not essential for contraction in these fibers. It has been also shown, by blocking Ica that Ica is not essential for triggering contraction (Gonzalez-Serratos et al., 1982). These results have led to the conclusion that Ica has no obvious role in E-C coupling in adult amphibian phasic skeletal muscle. The question arises then as to what is the biological role Ica in phasic skeletal muscle? We have observed that embryonic skeletal muscle cells are capable of contracting during the first day of development in culture (Cordoba-Rodriguez, et al., 1996), before the T-system and the sarcoplasmic reticulum (SR)may have fully developed (Flucher, et al., 1993).

Type
Philadelphia—The Other Motor City: Muscle and Non-Muscle Motility. A Dedication to Dr. Lee Peachey
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 92 - 93
Copyright
Copyright © Microscopy Society of America

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