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Recognition, Specificity, Scanning Probe Microscopy and Biomaterials

Published online by Cambridge University Press:  02 July 2020

Buddy D. Ratner ,
Reto Luginbühll ,
Rene Overney ,
Michael Garrison  and
Thomas Boland
Buddy D. Ratner
Affiliation:
Departments of Bioengineering University of Washington Engineered Biomaterials (UWEB) Box 351720, Seattle, Washington, 98195, USA
Reto Luginbühll
Affiliation:
Chemical Engineering University of Washington Engineered Biomaterials (UWEB) Box 351720, Seattle, Washington, 98195, USA
Rene Overney
Affiliation:
Chemical Engineering University of Washington Engineered Biomaterials (UWEB) Box 351720, Seattle, Washington, 98195, USA
Michael Garrison
Affiliation:
Chemical Engineering University of Washington Engineered Biomaterials (UWEB) Box 351720, Seattle, Washington, 98195, USA
Thomas Boland
Affiliation:
Chemical Engineering University of Washington Engineered Biomaterials (UWEB) Box 351720, Seattle, Washington, 98195, USA
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Abstract

Although scanning probe microscopy (SPM) can generate images of surface topography, this class of techniques is exceptionally valuable in its ability to provide quantitative and chemically specific information about biomaterial surfaces with high spatial definition. Since engineered biomaterials are designed to deliver chemically defined information, often arrayed in specific geometries, tools that can characterize such materials are needed.

A few years ago, we demonstrated how the atomic force microscope (AFM) could precisely distinguish between each of the four nucleotide bases that comprise DNA, measure the nucleotide-nucleotide force of interaction and spatially localize that information on a surface (1). in particular, we found that the nucleotide bases could self-assemble on gold. The assembly process was imaged using scanning tunneling microscopy (STM) and this led to an understanding of the structure of the assembled film. The assembled film structure was further characterized using electron spectroscopy for chemical analysis (ESCA) and secondary ion mass spectrometry (SIMS).

Type
Advances in Imaging Techniques for Biomaterlals (Organized by S. Eppel)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 130 - 131
Copyright
Copyright © Microscopy Society of America 2001

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References

references

(1)Boland, T; Ratner, BD (1995): Direct measurement by atomic force microscopy of hydrogen bonding in DNA nucleotide bases. Proc. Natl. Acad. Sci. USA 92(12), 5297–5301.Google Scholar
(2)Garrison, MD; Luginbuhl, R; Overney, RM; Ratner, BD (1998): Glow discharge plasma deposited hexafluoropropylene films: surface chemistry and interfacial materials properties. Thin Solid Films 352, 13–21.Google Scholar
(3)Pan, Y; Wesley, RA; Luginbuhl, R; Denton, DD; and Ratner, BD (2001); Plasma polymerized N-isopropylacrylamide: synthesis and characterization of a smart thermally responsive coating, Biomacromolecules, in press.CrossRefGoogle Scholar