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Quantitative Elemental Analysis of Heavy Metal Exposed Cells of Synechococcus Leopoliensis Using Regular and Overplus Cells: An Energy Disspersive X-Ray Spectroscopy Study

Published online by Cambridge University Press:  02 July 2020

J.J. Goldberg  and
T.E. Jensen
J.J. Goldberg
Affiliation:
Department of Biological Sciences, Lehman College and CUNY Grad. Center, Bronx, NY10468
T.E. Jensen
Affiliation:
Department of Biological Sciences, Lehman College and CUNY Grad. Center, Bronx, NY10468
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Extract

It has previously been shown that microbial polyphosphate bodies are the main sites of metal sequestration. In our present study, we have exposed Synechococcus leopoliensis, grown to log phase and to overplus phase, to five different heavy metals, Al, Cu, Cd, Pb, and Zn, independently. The cells were analyzed using the STEM mode of a transmission electron microscope in conjunction with a PGT IMIX energy dispersive x-ray spectrometer.

Cells of Synechococcus leopoliensis were grown for two weeks at 21°C on a 12-hour light/dark cycle in modified Fizgerald’s medium The second cell type were the overplus cells, which were grown as previously described. The cells were exposed to 100PPM of each metal for two hours, then were fixed and embedded in EPON according to Luff’s procedure.

For x-ray analysis, cells of interest were first located using the TEM mode. The microscope was then switched to the STEM mode.

Type
Mas Celebrates: Fifty Years of Electron Probe Microanalysis
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 586 - 587
Copyright
Copyright © Microscopy Society of America

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