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The Potential of Materials Analysis by Electron Rutherford Backscattering as Illustrated by a Case Study of Mouse Bones and Related Compounds

Published online by Cambridge University Press:  03 May 2013

Maarten Vos ,
Károly Tökési  and
Ilona Benkö
Maarten Vos*
Affiliation:
Research School of Physics and Engineering, Australian National University, Canberra, ACT 0200, Australia
Károly Tökési
Affiliation:
Institute of Nuclear Research, Hungarian Academy of Sciences (ATOMKI), P.O. Box 51, H-4001 Debrecen, Hungary
Ilona Benkö
Affiliation:
Department of Pharmacology and Pharmacotherapy, Medical and Health Science Center, University of Debrecen, P.O. Box 12, 4032 Debrecen, Hungary
*
*Corresponding author. E-mail: [email protected]
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Abstract

Electron Rutherford backscattering (ERBS) is a new technique that could be developed into a tool for materials analysis. Here we try to establish a methodology for the use of ERBS for materials analysis of more complex samples using bone minerals as a test case. For this purpose, we also studied several reference samples containing Ca: calcium carbonate (CaCO3) and hydroxyapatite and mouse bone powder. A very good understanding of the spectra of CaCO3 and hydroxyapatite was obtained. Quantitative interpretation of the bone spectrum is more challenging. A good fit of these spectra is only obtained with the same peak widths as used for the hydroxyapatite sample, if one allows for the presence of impurity atoms with a mass close to that of Na and Mg. Our conclusion is that a meaningful interpretation of spectra of more complex samples in terms of composition is indeed possible, but only if widths of the peaks contributing to the spectra are known. Knowledge of the peak widths can either be developed by the study of reference samples (as was done here) or potentially be derived from theory.

Keywords

elastic electron scatteringbone mineralelectron Rutherford backscatteringosteoporosiselemental analysiscalcium carbonatehydroxyapatite
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 3 , June 2013 , pp. 576 - 586
Copyright
Copyright © Microscopy Society of America 2013 

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